Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/874936
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dc.contributor.authorVALICENTE, F. H.pt_BR
dc.contributor.authorTUELHER, E. de S.pt_BR
dc.contributor.authorLEITE, M. I. S.pt_BR
dc.contributor.authorFREIRE, F. L.pt_BR
dc.contributor.authorVIEIRA, C. M.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2011-01-28pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationRevista Brasileira de Milho e Sorgo, Sete Lagoas, v. 9, n. 1, p. 1-11, 2010.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/874936pt_BR
dc.descriptionBacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBiocontrolept_BR
dc.subjectInseticida microbianopt_BR
dc.titleProduction of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-05-19T11:11:11Zpt_BR
dc.subject.thesagroPraga de plantapt_BR
dc.subject.thesagroInseticidapt_BR
dc.subject.thesagroSpodoptera Frugiperdapt_BR
riaa.ainfo.id874936pt_BR
riaa.ainfo.lastupdate2017-05-19pt_BR
dc.identifier.doihttp://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11pt_BR
dc.contributor.institutionFERNANDO HERCOS VALICENTE, CNPMS.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

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