Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/880372
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorBRANDALISE, M.pt_BR
dc.contributor.authorSEVERINO, F. E.pt_BR
dc.contributor.authorMALUF, M. P.pt_BR
dc.contributor.authorMAIA, I. G.pt_BR
dc.date.accessioned2011-04-09T22:27:47Z-
dc.date.available2011-04-09T22:27:47Z-
dc.date.created2011-03-10pt_BR
dc.date.issued2009pt_BR
dc.identifier.citationIn.: PLANT CELL REPORTS, v. 28, p. 1699-1708, 2009.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/880372pt_BR
dc.descriptionA cDNA clone (designated CaIRL) encoding an isoflavone reductase-like protein from coffee (Coffea arabica) was retrieved during a search for genes showing organ/tissue-specific expression among the expressed sequence tags (EST) of the Brazilian coffee EST database. The CaIRL cDNA contains a single open reading frame of 946 nucleotides (nt) encoding 314 amino acids (predicted molecular weight of 34 kDa). Several features identified the predicted CaIRL protein as a new member of the PIP family of NADPH-dependent reductases. Expression studies demonstrated that CaIRL is expressed exclusively in coffee leaves and its transcript level is markedly increased in response to fungal infection and mechanical injury. Analysis of transgenic tobacco plants harboring a CaIRL 50-flanking region (862 nt) fused to uidA reporter gene (GUS) confirmed the responsiveness of the putative promoter to abiotic stress in wounded leaves. In turn, a 50 deletion to -404 completely abolished promoter activation by abiotic stimulus in transgenic plants. The lack of GUS expression in non-wounded leaf tissues in transgenic tobacco was in contrast to the basal level of CaIRL expression observed in non-stressed healthy coffee leaves.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectTissue-specificitypt_BR
dc.subjectCoffeept_BR
dc.subjectIsoflavone-like proteinspt_BR
dc.subjectPromoterpt_BR
dc.titleThe promoter of a gene encoding an isoflavon reductase-like protein in coffee (Coffea arabica) drives a stress-responsive expression in leaves.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
dc.subject.nalthesaurusGene expressionpt_BR
riaa.ainfo.id880372pt_BR
riaa.ainfo.lastupdate2011-03-10pt_BR
dc.contributor.institutionMIRIAN PEREZ MALUF, SAPC.pt_BR
Aparece nas coleções:Artigo em periódico indexado (SAPC)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Thepromoter.pdf758,61 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace