Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/880493
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dc.contributor.authorBUDZINSKI, I. G. F.pt_BR
dc.contributor.authorSANTOS, T. B.pt_BR
dc.contributor.authorSERA, T.pt_BR
dc.contributor.authorPOT, D.pt_BR
dc.contributor.authorVIEIRA, L. G. E.pt_BR
dc.contributor.authorPEREIRA, L. F. P.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2011-03-11pt_BR
dc.date.issued2011pt_BR
dc.identifier.citationPlant Biology, Germany, v.13, n. 3, p. 462?471, 2011.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/880493pt_BR
dc.descriptionAs a first step towards understanding the physiological role and regulation of the expansin gene (EXP) family in Coffea arabica fruits during growth and maturation, we identified 11 expansin genes, nine belonging to the expansin family (EXPA), one EXLA and one EXLB, through in silico analysis of expressed sequence tags (ESTs). Within the expansin family, three isoforms were selected for detailed examination based on their high expression in coffee fruits or because they were specifically induced during different fruit developmental stages, according to the EST information. The expression patterns were analysed in different fruit tissues (perisperm, endosperm and pericarp) of C. arabica cv. IAPAR-59 and C. arabica cv. IAPAR-59 Grau´ do, the latter being a closely related cultivar with a larger fruit size. Accumulation of CaEXPA1 and CaEXPA3 transcripts was high in the perisperm (tissue responsible for coffee bean size) and in the early stages of pericarp development. Transcripts of CaEXPA2 were detected only in the pericarp during the later stages of fruit maturation and ripening. There was no detectable transcription of the three EXPs analysed in the endosperm. The observed differences in mRNA expression levels of CaEXPA1 and CaEXP3 in the perisperm of IAPAR-59 and IAPAR-59 Grau´do suggest the participation of these two isoforms in the regulation of grain size.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPerispermpt_BR
dc.subjectCoffeept_BR
dc.subjectExpasion isoformspt_BR
dc.subjectFruit ripeningpt_BR
dc.titleExpression patterns of three a-expansin isoforms in Coffea arabica during fruit development.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-10-04T11:11:11Zpt_BR
dc.subject.nalthesaurusGrain qualitypt_BR
riaa.ainfo.id880493pt_BR
riaa.ainfo.lastupdate2011-10-04pt_BR
dc.contributor.institutionIAPAR; IAPAR/Universidade Estadual de Londrina; IAPAR; CIRAD/UMR/DAP; IAPAR; LUIZ FILIPE PROTASIO PEREIRA, SAPC.pt_BR
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