Título:	Genetic diversity in cultivated carioca common beans based on molecular marker analysis.
Autoria:	PERSEGUINI, J. M. C. K. CHIORATTO, A. F. ZUCCHI, M. I. COLOMBO, C. A. CARBONELL, S. A. M. MONDEGO, J. M. C. GAZAFFI, R. GARCIA, A. A. F. CAMPOS, T. de SOUZA, A. P. de RUBIANO, L. B.
Afiliação:	Juliana Morini Küpper Cardoso Perseguini, IAC; Alisson F ernando Chioratto, IAC; Maria Imaculada Zucchi, IAC; Carlos Augusto Colombo, IAC; Sérgio Augusto Moraes Carbonell, IAC; Jorge Mauricio Costa Mondego, IAC; Rodrigo Gazaffi, ESALQ; Antonio Augusto Franco Garcia, ESALQ; TATIANA DE CAMPOS, CPAF-AC; Anete Pereira de Souza, UNICAMP; Luciana Benchimol Rubiano, IAC.
Ano de publicação:	2011
Referência:	Genetics and Molecular Biology, Ribeirão Preto, v. 34, n. 1, p. 88-102, 2011.
Conteúdo:	A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger?s modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were polymorphic and 20 AFLP primer combinations produced 635 polymorphic bands. Molecular analysis showed that carioca genotypes were quite diverse. AFLPs revealed greater genetic differentiation and variation within the carioca genotypes (Gst = 98% and Fst = 0.83, respectively) than SSRs and provided better resolution for clustering the carioca genotypes. SSRs and AFLPs were both suitable for assessing the genetic diversity of Brazilian carioca genotypes since the number of markers used in each system provided a low coefficient of variation. However, fingerprint profiles were generated faster with AFLPs, making them a better choice for assessing genetic diversity in the carioca germplasm.
Thesagro:	Melhoramento genético vegetal Feijão Phaseolus vulgaris Variação genética Marcador genético Polimorfismo genético
NAL Thesaurus:	Plant breeding Beans Genetic variation Genetic markers Microsatellite repeats Genetic polymorphism
Palavras-chave:	Marcador microssatélite Feijão carioca Frijoles Fitomejoramiento Marcadores genéticos Repeticiones de microsatélite Variación genética
Digital Object Identifier:	10.1590/S1415-47572011000100017
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPAF-AC)