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dc.contributor.authorMISSIO, R. F.pt_BR
dc.contributor.authorCAIXETA, E. T.pt_BR
dc.contributor.authorZAMBOLIM, E. M.pt_BR
dc.contributor.authorPENA, G. F.pt_BR
dc.contributor.authorZAMBOLIM, L.pt_BR
dc.contributor.authorDIAS, L. A. S.pt_BR
dc.contributor.authorSAKIYAMA, N. S.pt_BR
dc.date.accessioned2011-10-18T11:11:11Zpt_BR
dc.date.accessioned2011-10-18T11:11:11Zpt_BR
dc.date.available2011-10-18T11:11:11Zpt_BR
dc.date.available2011-10-18T11:11:11Zpt_BR
dc.date.created2011-10-18pt_BR
dc.date.issued2011pt_BR
dc.identifier.citationGenetics and Molecular Research, v. 10, n. 4, p 2366-2381, 2011.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/903366pt_BR
dc.descriptionCoffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), resistant arabica (six leaf rust-resistant C. arabica genotypes with introgression of Híbrido de Timor), robusta (five C. canephora genotypes), Híbrido de Timor (three C. arabica x C. canephora), triploids (three C. arabica x C. racemosa), and racemosa (one C. racemosa). Allele and polymorphism analysis, AMOVA, the Student t-test, Jaccard?s dissimilarity coefficient, cluster analysis, correlation of genetic distances, and discriminant analysis, were performed. EST-SSR markers gave 25 exclusive alleles per genetic group, while gSSR showed 47, which will be useful for differentiating accessions and for fingerprinting varieties. The gSSR markers detected a higher percentage of polymorphism among (35% higher on average) and within (42.9% higher on average) the genetic groups, compared to EST-SSR markers. The highest percentage of polymorphism within the genetic groups was found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%). It was possible to differentiate all genotypes including the arabica-related accessions. Nevertheless, combined use of gSSR and EST-SSR markers is recommended for coffee molecular characterization, because EST-SSRs can provide complementary information.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGenetic diversitypt_BR
dc.subjectMicrosatellite markerpt_BR
dc.titleGenetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-10-18T11:11:11Zpt_BR
dc.subject.nalthesaurusCoffeapt_BR
dc.subject.nalthesaurusDiscriminant analysispt_BR
riaa.ainfo.id903366pt_BR
riaa.ainfo.lastupdate2011-10-18pt_BR
dc.contributor.institutionUniversidade Federal do Paraná; EVELINE TEIXEIRA CAIXETA, SAPC; BioCafé; BioCafé; BioCafé; UFV; BioCafé/UFV.pt_BR
Aparece nas coleções:Artigo em anais de congresso (SAPC)

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