Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/908752
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dc.contributor.authorMOURÃO, M. M.pt_BR
dc.contributor.authorBITAR, M. L.pt_BR
dc.contributor.authorLOBO, F. P.pt_BR
dc.contributor.authorGRYNBERG, P.pt_BR
dc.contributor.authorBORONI, M.pt_BR
dc.contributor.authorWAISBERG, M.pt_BR
dc.contributor.authorMACEDO, A. M.pt_BR
dc.contributor.authorMACHADO, C. R.pt_BR
dc.contributor.authorFRANCO, G. R.pt_BR
dc.date.accessioned2011-12-06T11:11:11Zpt_BR
dc.date.accessioned2011-12-06T11:11:11Zpt_BR
dc.date.available2011-12-06T11:11:11Zpt_BR
dc.date.available2011-12-06T11:11:11Zpt_BR
dc.date.created2011-12-06pt_BR
dc.date.issued2011pt_BR
dc.identifier.citationIn: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional, 2011.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/908752pt_BR
dc.descriptionSchistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstrated that trans-splicing knockdown in sprocysts caused a reduction on the levels of all tested trans-spliced transcripts, but surprisingly the only phenotypic effect observed was a diminished larvae size. Financial support: CNPq, FAPEMIGpt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectExpressão gênicapt_BR
dc.titleRevealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Shistosoma mansoni.pt_BR
dc.typeResumo em anais e proceedingspt_BR
dc.date.updated2020-01-24T11:11:11Zpt_BR
dc.subject.thesagroSchistosoma Mansonipt_BR
dc.subject.nalthesaurusGene expressionpt_BR
dc.description.notesX-MEETING 2011.pt_BR
dc.format.extent2Não paginado.pt_BR
riaa.ainfo.id908752pt_BR
riaa.ainfo.lastupdate2020-01-24 -02:00:00pt_BR
dc.contributor.institutionMARINA MORAES MOURÃO, FIOCRUZ; MAINÁ LAST BITAR, UFMG, NUBIO; FRANCISCO PEREIRA LOBO, CNPTIA; PRISCILA GRYNBERG, UFMG; MARIANA BORONI, UFMG; MICHAEL WAISBERG, NIH; ANDRÉA MARA MACEDO, UFMG; CARLOS RENATO MACHADO, UFMG; GLÓRIA REGINA FRANCO, UFMG.pt_BR
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