Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/932471
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorFRANÇA, C. A.pt_BR
dc.contributor.authorPEIXOTO, R. M.pt_BR
dc.contributor.authorCAVALCANTE, M. B.pt_BR
dc.contributor.authorMELO, N. F. dept_BR
dc.contributor.authorOLIVEIRA, C. J. B.pt_BR
dc.contributor.authorVESCHI, J. L. A.pt_BR
dc.contributor.authorMOTA, R. A.pt_BR
dc.contributor.authorCOSTA, M. M.pt_BR
dc.date.accessioned2012-08-28T11:11:11Zpt_BR
dc.date.available2012-08-28T11:11:11Zpt_BR
dc.date.created2012-08-28pt_BR
dc.date.issued2012pt_BR
dc.identifier.citationPesquisa Veterinária Brasileira, v. 32, n. 8, p. 747-753, ago. 2012.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/932471pt_BR
dc.descriptionThe study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efflux pump test was performed using ethidium bromide and biofilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-sus- ceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efflux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isola - tes presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efflux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBomba de efluxopt_BR
dc.subjectMastitept_BR
dc.titleAntimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2012-08-28T11:11:11Zpt_BR
dc.subject.thesagroCaprinopt_BR
dc.subject.thesagroOvinopt_BR
dc.subject.thesagroBiofilmept_BR
dc.subject.thesagroCabrapt_BR
dc.subject.thesagroLeitept_BR
dc.subject.nalthesaurusGoatspt_BR
dc.subject.nalthesaurusSheeppt_BR
dc.subject.nalthesaurusStaphylococcuspt_BR
riaa.ainfo.id932471pt_BR
riaa.ainfo.lastupdate2012-08-28pt_BR
dc.contributor.institutionCHIRLES A. FRANÇA; RODOLFO M. PEIXOTO, Instituto Federal de Educação, Ciência e Tecnologia do Sertão Pernambucano, Campus Floresta; MARIELLY B. CAVALCANTE; NATONIEL FRANKLIN DE MELO, CPATSA; CELSO JOSÉ B. OLIVEIRA, UFPB; JOSIR LAINE APARECIDA VESCHI, CPATSA; RINALDO A. MOTA, UFRPE; MATEUS M. COSTA, UNIVASF.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CPATSA)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Natoniel.pdf328,15 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace