Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/938024
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dc.contributor.authorSILVEIRA, E. D.
dc.contributor.authorGUIMARÃES, L. A.
dc.contributor.authorDUSI, D. M. de A.
dc.contributor.authorSILVA, F. R. da
dc.contributor.authorMARTINS, N. F.
dc.contributor.authorCOSTA, M. M. do C.
dc.contributor.authorALVES-FERREIRA, M.
dc.contributor.authorCARNEIRO, V. T. de C.
dc.date.accessioned2018-06-13T00:46:15Z-
dc.date.available2018-06-13T00:46:15Z-
dc.date.created2012-10-25
dc.date.issued2012
dc.identifier.citationPlant Cell Reports, v. 31, p. 403-416, 2012.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/938024-
dc.descriptionAbstract - In apomixis, asexual mode of plant reproduction through seeds, an unreduced megagametophyte is formed due to circumvented or altered meiosis. The embryo develops autonomously from the unreduced egg cell, independently of fertilization. Brachiaria is a genus of tropical forage grasses that reproduces sexually or by apomixis. A limited number of studies have reported the sequencing of apomixis-related genes and a few Brachiaria sequences have been deposited at genebank databases. This work shows sequencing and expression analyses of expressed sequence-tags (ESTs) of Brachiaria genus and points to transcripts from ovaries with preferential expression at megasporogenesis in apomictic plants. From the 11 differentially expressed sequences from immature ovaries of sexual and apomictic Brachiaria brizantha obtained from macroarray analysis, 9 were preferentially detected in ovaries of apomicts, as con?rmed by RT-qPCR. A putative involvement in early steps of Panicum-type embryo sac differentiation of four sequences from B. brizantha ovaries: BbrizHelic, BbrizRan, BbrizSec13 and BbrizSti1 is suggested. Two of these, BbrizSti1 and BbrizHelic, with similarity to a gene coding to stress induced protein and a helicase, respectively, are preferentially expressed in the early stages of apomictic ovaries development, especially in the nucellus, in a stage previous to the differentiation of aposporous initials, as veri?ed by in situ hybridization.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectForage grass
dc.titleExpressed sequence-tag analysis of ovaries of Brachiaria brizantha reveals genes associated with the early steps of embryo sac differentiation of apomictic plants.
dc.typeArtigo de periódico
dc.date.updated2018-06-13T00:46:15Zpt_BR
dc.subject.nalthesaurusApomixis
dc.subject.nalthesaurusPlant reproduction
riaa.ainfo.id938024
riaa.ainfo.lastupdate2018-06-12
dc.contributor.institutionÉRICA DUARTE SILVEIRA, UFRJ; LARISSA ARRAIS GUIMARÃES, UNB; DIVA MARIA DE ALENCAR DUSI, CENARGEN; FELIPE RODRIGUES DA SILVA, CNPTIA; NATALIA FLORENCIO MARTINS, CENARGEN; MARCOS MOTA DO CARMO COSTA, CENARGEN; MÁRCIO ALVES-FERREIRA, UFRJ; VERA TAVARES DE CAMPOS CARNEIRO, CENARGEN.
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