Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/940523
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dc.contributor.authorMORALES, A.pt_BR
dc.contributor.authorO'ROURKE, J. A.pt_BR
dc.contributor.authorBORÉM, A.pt_BR
dc.contributor.authorABDELNOOR, R. V.pt_BR
dc.contributor.authorPEDLEY, K. F.pt_BR
dc.contributor.authorWHITHAM, S. A.pt_BR
dc.contributor.authorGRAHAM, M. A.pt_BR
dc.date.accessioned2012-11-26T11:11:11Zpt_BR
dc.date.available2012-11-26T11:11:11Zpt_BR
dc.date.created2012-11-26pt_BR
dc.date.issued2012pt_BR
dc.identifier.citationIn: MOLECULAR & CELLULAR BIOLOGY OF TE SOYBEAN, 14., Des Moines, 2012. [Proceedings...]. Des Moines: Iowa State University, 2012.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/940523pt_BR
dc.descriptionSix Asian Soybean Rust (ASR) resistance loci have been identified and mapped in soybean genome: Rpp1 (Resistance to Phakopsora pachyrhizi 1), Rpp2, Rpp3, Rpp4, Rpp5 and Rpp6. Of particular interest is Rpp4, which has remained stable and confers resistance against Phakopsora pachyrhizi isolates from around the world. Sequencing of the region harboring Rpp4 in the susceptible cultivar Williams 82 (Wm82) and the resistant cultivar (PI459025B) genotype identified a cluster of CC-NBS-LRR resistance genes. Meyers et al. (2009) developed Virus Induced Gene Silencing constructs from the LRR regions of the Wm82 Rpp4 candidate genes to confirm that orthologous genes were responsible for resistance in the resistant parent (PI459025B). In this study, RNA samples extracted from the same Rpp4 LRR silenced and empty vector treated plants, also infected with P. pachyrhizi (described by Meyer et al., 2009) were compared using the GeneChip® Soybean Genome Array (Affymetrix®). Since the plant samples differed only in the expression of Rpp4, comparisons of these samples would identify genes downstream of Rpp4 in the signaling pathway. In total, 383 differentially expressed probes were identified, many with functions related to defense. While the time point analyzed was late in defense signaling, bioinformatic approaches were useful in characterizing the defense response and identify transcription factors regulating the response.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleCombining transcriptome analyses and virus induced gene silencing to identify genes in the Rpp4-mediated asian soybean rust resistance Pathway.pt_BR
dc.typeResumo em anais e proceedingspt_BR
dc.date.updated2018-04-16T11:11:11Zpt_BR
dc.subject.thesagroSojapt_BR
dc.subject.thesagroDoença de plantapt_BR
dc.subject.thesagroFerrugempt_BR
dc.subject.thesagroPhakopsora Pachyrhizipt_BR
dc.subject.nalthesaurusSoybeanspt_BR
dc.subject.nalthesaurusRust diseasespt_BR
dc.subject.nalthesaurusPlant diseases and disorderspt_BR
dc.format.extent2p. 137.pt_BR
riaa.ainfo.id940523pt_BR
riaa.ainfo.lastupdate2018-04-16 -03:00:00pt_BR
dc.contributor.institutionAGUIDA MORALES; JAMIE A.O'ROURKE, USDA-ARS; ALUÍZIO BORÉM, UFV; RICARDO VILELA ABDELNOOR, CNPSO; KERRY F. PEDLEY, USDA-ARS; STEVEN A. WHITHAM, IOWA STATE UNIVERSITY; MICHELLE A. GRAHAM, USDA-ARS.pt_BR
Aparece nas coleções:Resumo em anais de congresso (CNPSO)

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