Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/974120
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dc.contributor.authorCOSTA, S. R. dapt_BR
dc.contributor.authorSANTOS, C. A. F.pt_BR
dc.date.accessioned2013-12-17T11:11:11Zpt_BR
dc.date.available2013-12-17T11:11:11Zpt_BR
dc.date.created2013-12-17pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationGenetics and Molecular Research, v. 12, n. 4, p. 6802-6812, 2013.pt_BR
dc.identifier.issn1676-5680pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/974120pt_BR
dc.descriptionThis study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variability is not uniformly distributed among the 9 Brazilian states from where the accession were obtained. Obtaining a greater number of accessions by Brazilian states is recommended in order to have greater diversity among the species.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectVariabilidade genéticapt_BR
dc.subjectMarcadores microssatélitespt_BR
dc.subjectBanco ativo de germoplasmapt_BR
dc.subjectDendrogramapt_BR
dc.subjectMelhoramento genéticopt_BR
dc.subjectGuavapt_BR
dc.titleAllelic database and divergence among Psidium accessions by using microsatellite markers.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2013-12-17T11:11:11Zpt_BR
dc.subject.thesagroGoiabapt_BR
dc.subject.nalthesaurusBreedingpt_BR
dc.subject.nalthesaurusPsidiumpt_BR
riaa.ainfo.id974120pt_BR
riaa.ainfo.lastupdate2013-12-17pt_BR
dc.contributor.institutionS. R. da COSTA, Universidade Estadual de Feira de Santana, Feira de Santana, BApt_BR
dc.contributor.institutionCARLOS ANTONIO FERNANDES SANTOS, CPATSA.eng
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