Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/977492
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dc.contributor.authorCOSTA, F. M.pt_BR
dc.contributor.authorSCHIAVO, J. A.pt_BR
dc.contributor.authorBRASIL, M. S.pt_BR
dc.contributor.authorLEITE, J.pt_BR
dc.contributor.authorXAVIER, G. R.pt_BR
dc.contributor.authorFERNANDES JUNIOR, P. I.pt_BR
dc.date.accessioned2014-01-27T11:11:11Zpt_BR
dc.date.available2014-01-27T11:11:11Zpt_BR
dc.date.created2014-01-27pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationGenetics and Molecular Research, v. 13, n. 1, p. 469-482 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/977492pt_BR
dc.descriptionThe aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, and amylolytic and cellulolytic activities. The molecular characterization of the bacterial isolates was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and Box-polymerase chain reaction (PCR) techniques. In addition, the symbiotic performance of selected rhizobial isolates was evaluated in a greenhouse experiment using sterile substrate. The phenotypic characterization revealed that the bacterial strains obtained from pigeonpea root nodules presented characteristics that are uncommon among rhizobial isolates, indicating the presence of new species nodulating the pigeonpea plants in the Brazilian Pantanal. The molecular fingerprinting of these bacterial isolates also showed a highly diverse collection, with both techniques revealing less than 25% similarity among bacterial isolates. The evaluation of symbiotic performance also indicated the presence of microorganisms with high potential to increase the growth and nitrogen content at the shoots of pigeonpea plants. The results obtained in this study indicate the presence of a highly diversified rhizobial community nodulating the pigeonpea at the eastern edge of the Brazilian Pantanal.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectFeijão guandupt_BR
dc.subjectFixação biológica de nitrogêniopt_BR
dc.subjectEcologia microbianapt_BR
dc.subjectDiversidadept_BR
dc.subjectRizóbiopt_BR
dc.subjectNódulos radicularespt_BR
dc.titlePhenotypic and molecular finger printing of fast growing rhizobia of field-grown pigeonpea from the eastern edge of the Brazilian Pantanal.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-03-26T11:11:11Zpt_BR
dc.subject.thesagroFeijãopt_BR
dc.subject.thesagroBactériapt_BR
dc.subject.thesagroFixação de Nitrogêniopt_BR
dc.subject.nalthesaurusPlant geneticseng
dc.subject.nalthesaurusBeanseng
dc.subject.nalthesaurusGenetic disorderseng
riaa.ainfo.id977492pt_BR
riaa.ainfo.lastupdate2015-03-26pt_BR
dc.contributor.institutionUniversidade Estadual do Mato Grosso do Sul; Universidade Estadual do Mato Grosso do Sul; Universidade Federal do Mato Grosso do Sul; Universidade Federal Rural do Rio de Janeiro; GUSTAVO RIBEIRO XAVIER, CNPAB; PAULO IVAN FERNANDES JUNIOR, CPATSA.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CPATSA)

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