Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/980419
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dc.contributor.authorDALCIN, L.
dc.contributor.authorSILVA, R. C.
dc.contributor.authorPAULINI, F.
dc.contributor.authorSILVA, B. D. M.
dc.contributor.authorNEVES, J. P.
dc.contributor.authorLUCCI, C. M.
dc.date.accessioned2018-07-19T00:58:01Z-
dc.date.available2018-07-19T00:58:01Z-
dc.date.created2014-02-18
dc.date.issued2013
dc.identifier.citationCryobiology, v.67, n. 2, p. 137-145, 2013.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/980419-
dc.descriptionEven though sheep embryo cryopreservation is a commonly used procedure the survival and pregnancy outcomes can vary greatly. This study investigated whether cryopreservation was causing subtle changes in ultrastructure, mitochondrial activity or cytoskeletal integrity. Sheep embryos were either slow cooled in 1.5 M EG (n = 22), or vitrified in 20% EG + 20% DMSO with 0.5 M sucrose in Open Pulled Straws (OPS) (n = 24). One hour after warming the cryopreserved embryos differed from control embryos in that they had no mitochondrial activity combined with cytoskeletal disorganization and large vesicles. Vitrified embryos also showed many points of cytoskeleton disruption. Ultrastructural alterations resulting from actin filaments disorganization were observed in both cryopreserved groups. This includes areas presenting no cytoplasmic organelles, Golgi complex located far from the nucleus and a decrease of specialized intercellular junctions. Additionally, large vesicles were observed in vitrified morulae and early blastocysts. The alterations after cryopreservation were proportional to embryo quality as assessed using the stereomicroscope. Even in the absence of mitochondrial activity, grade I and II cryopreserved embryos contained mitochondria with normal ultrastructure. Embryos classified as grade I or II in the stereomicroscope revealed mild ultrastructural alterations, meaning that this tool is efficient to evaluate embryos after cryopreservation.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCongelamento lento
dc.subjectVitrificação
dc.subjectMicroscopia eletrônica de transmissão
dc.subjectMicroscopia confocal
dc.subjectOrganelas
dc.titleCytoskeleton structure, pattern of mitochondrial activity and ultrastructure of frozen or vitrified sheep embryos.
dc.typeArtigo de periódico
dc.date.updated2018-07-19T00:58:01Zpt_BR
riaa.ainfo.id980419
riaa.ainfo.lastupdate2018-07-18
dc.contributor.institutionUnB; UnB; UnB; BIANCA DAMIANI MARQUES SILVA, CENARGEN; UnB; UnB.
Aparece nas coleções:Artigo em periódico indexado (CENARGEN)

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