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dc.contributor.authorSILVA, G. T.pt_BR
dc.contributor.authorSILVA, C. B. C.pt_BR
dc.contributor.authorJANK, L.pt_BR
dc.contributor.authorSOUZA, A. P.pt_BR
dc.date.accessioned2014-02-27T11:11:11Zpt_BR
dc.date.available2014-02-27T11:11:11Zpt_BR
dc.date.created2014-02-27pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationPLOS ONE, v. 8, n. 7, p. 1-10, july 2013pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/981465pt_BR
dc.descriptionGuinea grass (Panicum maximum Jacq.) is a tropical African grass often used to feed beef cattle, which is an important economic activity in Brazil. Brazil is the leader in global meat exportation because of its exclusively pasture-raised bovine herds. Guinea grass also has potential uses in bioenergy production due to its elevated biomass generation through the C4 photosynthesis pathway. We generated approximately 13 Gb of data from Illumina sequencing of P. maximum leaves. Four different genotypes were sequenced, and the combined reads were assembled de novo into 38,192 unigenes and annotated; approximately 63% of the unigenes had homology to other proteins in the NCBI non-redundant protein database. Functional classification through COG (Clusters of Orthologous Groups), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses showed that the unigenes from Guinea grass leaves are involved in a wide range of biological processes and metabolic pathways, including C4 photosynthesis and lignocellulose generation, which are important for cattle grazing and bioenergy production. The most abundant transcripts were involved in carbon fixation, photosynthesis, RNA translation and heavy metal cellular homeostasis. Finally, we identified a number of potential molecular markers, including 5,035 microsatellites (SSRs) and 346,456 single nucleotide polymorphisms (SNPs). To the best of our knowledge, this is the first study to characterize the complete leaf transcriptome of P. maximum using highthroughput sequencing. The biological information provided here will aid in gene expression studies and marker-assisted selection-based breeding research in tropical grasses.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleDe novo transcriptome assembly for the tropical grass Panicum maximum Jacq.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-02-21T11:11:11Zpt_BR
dc.subject.thesagroPanicum maximumpt_BR
dc.subject.thesagroAlimento animalpt_BR
dc.subject.thesagroGraminea tropicalpt_BR
dc.subject.thesagroCarnept_BR
dc.subject.nalthesaurusBeef cattlept_BR
dc.subject.nalthesaurusMegathyrsus maximuspt_BR
riaa.ainfo.id981465pt_BR
riaa.ainfo.lastupdate2018-02-21 -03:00:00pt_BR
dc.contributor.institutionGUILHERME TOLEDO-SILVA, Molecular Biology Center and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.pt_BR
dc.contributor.institutionCLAUDIO BENICIO CARDOSO-SILVA, Molecular Biology Center and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazileng
dc.contributor.institutionLIANA JANK, CNPGCeng
dc.contributor.institutionANETE PEREIRA SOUZA, Molecular Biology Center and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, São Paulo, Brazileng
dc.contributor.institutionDepartment of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, São Paulo, BraziL.eng
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