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http://www.alice.cnptia.embrapa.br/alice/handle/doc/985516
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Campo DC | Valor | Idioma |
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dc.contributor.author | MOURA, R. R. | pt_BR |
dc.contributor.author | SOUZA-FABJAN, J. M. G. | pt_BR |
dc.contributor.author | FONSECA, J. F. da | pt_BR |
dc.contributor.author | MELO, C. H. S. | pt_BR |
dc.contributor.author | SANCHEZ, D. J. D. | pt_BR |
dc.contributor.author | VIEIRA, M. P. | pt_BR |
dc.contributor.author | ALMEIDA, T. M. | pt_BR |
dc.contributor.author | SEROVA, I. A. | pt_BR |
dc.contributor.author | SEROV, O. L. | pt_BR |
dc.contributor.author | PEREIRA, A. F. | pt_BR |
dc.contributor.author | TEIXEIRA, D. I. A. | pt_BR |
dc.contributor.author | MELO, L. M. | pt_BR |
dc.contributor.author | FREITAS, V. J. F. | pt_BR |
dc.date.accessioned | 2014-05-02T11:11:11Z | pt_BR |
dc.date.available | 2014-05-02T11:11:11Z | pt_BR |
dc.date.created | 2014-05-02 | pt_BR |
dc.date.issued | 2014 | pt_BR |
dc.identifier.citation | Animal Reproduction, v. 11, n. 1, p. 37-43, Jan./Mar. 2014. | pt_BR |
dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/985516 | pt_BR |
dc.description | Abstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene. | pt_BR |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Transgenia | pt_BR |
dc.subject | Goat | pt_BR |
dc.title | Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene. | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.date.updated | 2014-05-02T11:11:11Z | pt_BR |
dc.subject.thesagro | Caprino | pt_BR |
dc.subject.thesagro | Cabra | pt_BR |
dc.subject.thesagro | Transferência de embrião | pt_BR |
dc.subject.thesagro | Hormônio animal | pt_BR |
dc.subject.thesagro | Progesterona | pt_BR |
dc.subject.thesagro | Superovulação | pt_BR |
dc.subject.nalthesaurus | Embryo transfer | pt_BR |
dc.subject.nalthesaurus | Progesterone | pt_BR |
dc.subject.nalthesaurus | Superovulation | pt_BR |
dc.subject.nalthesaurus | Transgenesis | pt_BR |
riaa.ainfo.id | 985516 | pt_BR |
riaa.ainfo.lastupdate | 2014-05-02 | pt_BR |
dc.contributor.institution | Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; Institute of Cytology and Genetics, Novosibirsk, Russia.; Institute of Cytology and Genetics, Novosibirsk, Russia.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil. | pt_BR |
Aparece nas coleções: | Artigo em periódico indexado (CNPC) |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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APIReproductiveparameters.pdf | 421,97 kB | Adobe PDF | Visualizar/Abrir |