Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/986801
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dc.contributor.authorNICKEL, O.pt_BR
dc.contributor.authorFAJARDO, T. V. M.pt_BR
dc.date.accessioned2016-02-22T12:37:55Z-
dc.date.available2016-02-22T12:37:55Z-
dc.date.created2014-05-22pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationJournal of Plant Pathology, v. 96, n. 1, p. 207-213, 2014.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/986801pt_BR
dc.descriptionApple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV) are common in apples and pears and the main targets of virus elimination from propagation material. The objective of this work was to design primers and probes for a real time RT-PCR protocol for detection of the four above viruses. FAM/TAMRA-labeled probes and primers were designed by searching for highly conserved nucleotide regions in the coat protein gene of the four viruses. Infection levels in analyzed apple samples were 92.6, 96.4, 100 and 88% for ASGV, ASPV, ACLSV and ApMV, respectively. In pears, all pre-existing ASPV infections were detected. Viral infections were confirmed in a selection of commercial cultivars of apples and pear scions, and quince rootstocks, demonstrating the sensitivity and reliability of the designed primers and probes. Real time RT-PCR using 5'-labeled probes is suitable for checking sanitary quality as a routine test in certification programs.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectRT-PCRpt_BR
dc.titleDetection of Viruses in Apples and Pears by Real Time RT-PCR Using 5'-Hydrolysis Probespt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2019-04-02T11:11:11Zpt_BR
dc.subject.thesagroMaçapt_BR
dc.subject.thesagroPerapt_BR
dc.subject.thesagroViruspt_BR
dc.subject.thesagroDNApt_BR
dc.subject.nalthesaurusApple chlorotic leaf spot viruspt_BR
dc.subject.nalthesaurusApple mosaic viruspt_BR
dc.subject.nalthesaurusApple stem pitting viruspt_BR
dc.subject.nalthesaurusApple stem grooving viruspt_BR
dc.subject.nalthesaurusReverse transcriptase polymerase chain reactionpt_BR
dc.subject.nalthesaurusFruit qualitypt_BR
riaa.ainfo.id986801pt_BR
riaa.ainfo.lastupdate2019-04-02 -03:00:00pt_BR
dc.contributor.institutionOSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPUV)

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