Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/988256
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dc.contributor.authorSILVA, C. C.pt_BR
dc.contributor.authorMANTELLO, C. C.pt_BR
dc.contributor.authorCAMPOS, T. dept_BR
dc.contributor.authorSOUZA, L. M.pt_BR
dc.contributor.authorGONÇALVES, P. S.pt_BR
dc.contributor.authorSOUZA, A. P.pt_BR
dc.date.accessioned2014-06-11T11:11:11Zpt_BR
dc.date.available2014-06-11T11:11:11Zpt_BR
dc.date.created2014-06-11pt_BR
dc.date.issued2014pt_BR
dc.identifier.citationMolecular Breeding, Berlim, v. 34, n. 3, p. 1035-1053, Oct. 2014.pt_BR
dc.identifier.issn1380-3743 (impresso) / 1572-9788 (on-line)pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/988256pt_BR
dc.descriptionHevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMarcador SNPpt_BR
dc.subjectRubber treept_BR
dc.subjectMarcador microssatélitept_BR
dc.subjectBiblioteca cDNApt_BR
dc.subjectCDNA librarypt_BR
dc.subjectFitomejoramientopt_BR
dc.subjectMarcadores genéticospt_BR
dc.subjectRepeticiones de microsatélitept_BR
dc.titleLeaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2019-10-29T11:11:11Zpt_BR
dc.subject.thesagroMelhoramento genético vegetalpt_BR
dc.subject.thesagroSeringueirapt_BR
dc.subject.thesagroHevea brasiliensispt_BR
dc.subject.thesagroMarcador genéticopt_BR
dc.subject.thesagroDNApt_BR
dc.subject.thesagroCauchopt_BR
dc.subject.nalthesaurusPlant breedingpt_BR
dc.subject.nalthesaurusGenetic markerspt_BR
dc.subject.nalthesaurusMicrosatellite repeatspt_BR
riaa.ainfo.id988256pt_BR
riaa.ainfo.lastupdate2019-10-29 -03:00:00pt_BR
dc.identifier.doi10.1007/s11032-014-0095-2pt_BR
dc.contributor.institutionCarla C. Silva, Universidade Estadual de Campinas (Unicamp); Camila C. Mantello, Universidade Estadual de Campinas (Unicamp); TATIANA DE CAMPOS, CPAF-AC; Livia M. Souza, Universidade Estadual de Campinas (Unicamp); Paulo S. Gonçalves, Instituto Agronômico de Campinas (IAC); Anete P. Souza, Universidade Estadual de Campinas (Unicamp).pt_BR
Aparece nas coleções:Artigo em periódico indexado (CPAF-AC)

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