Title:	Linearization of the Bradford protein assay to application in cow milk proteins quantification by UV-Vis spectrophotometry method.
Authors:	SANTOS, A. S. de O. dos COSTA, F. F. ESTEVES, W. T. BRITO, M. A. V. P. e FURTADO, M. A. M. MARTINS, M. F.
Affiliation:	ALESSA SIQUEIRA DE OLIVEIRA DOS SANTOS; FABIANO FREIRE COSTA, UFJF; WESLEY TINOCO ESTEVES; MARIA APARECIDA V PAIVA E BRITO, CNPGL; MARCO ANTÔNIO MOREIRA FURTADO, UFJF; MARTA FONSECA MARTINS, CNPGL.
Date Issued:	2014
Citation:	Revista do Instituto de Laticínios Cândido Tostes, v. 69, n. 6, p. 415-423, 2014.
Description:	Reliable methods for determination and quantification of total protein in food are essential information to ensure quality and safety of food trade. The objective of this study was to evaluate the linearity of calibration curves obtained from different proteins (blood serum albumin-BSA, α-LA, β-LG, caseins (CN): αs, β and κ-CAS) with the reagent of Bradford. Comercial UHT skimmed bovine milk was analyzed for the determination of total protein using the Bradford method by reading at 595 nm. The determination of the concentrations of total milk protein was achieved by linear regression. The Bradford method showed a high sensitivity for the determination of total proteins in bovine milk dilution 1:25 to values closer to those obtained by the Kjeldahl method. The results showed that the calibration curve of standard proteins β-CN and BSA obtained better linearity with less variation in the absorbance measurements for the determination of total protein of milk.
NAL Thesaurus:	beta-casein
Keywords:	Bovine Skimmed milk Spectrophotometry Proteins quantification BSA
DOI:	https://doi.org/10.14295/2238-6416.v69i6.350
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)