Title:	A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.
Authors:	MARTINS, P. K. RIBEIRO, A. P. DIAS, B. B. A. KOBAYASHI, A. K. MOLINARI, H. B. C.
Affiliation:	Polyana Kelly Martins; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE.
Date Issued:	2015
Citation:	Biotechnology Reports, v. 6, p. 41-44, 2015.
Pages:	p. 41-44
Description:	Abstract: The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for Agrobacterium-mediated genetic transformation of S. viridis. The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane
Thesagro:	Cana de Açúcar Bioenergia Milho Verde Genoma
NAL Thesaurus:	Bioenergy Sugarcane Setaria viridis Genomics
Keywords:	Model system C4 metabolism Green millet
DOI:	https://doi.org/10.1016/j.btre.2015.02.002
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPAE)