Título:	Galactinol synthase transcriptional profile in two genotypes of Coffea canephora with contrasting tolerance to drought.
Autoria:	SANTOS, T. B. dos LIMA, R. B. de NAGASHIMA, G. T. PETKOWICZ, C. L. de O. CARPENTIERI-PÍPOLO, V. PEREIRA, L. F. P. DOMINGUES, D. S. VIEIRA, L. G. E.
Afiliação:	TIAGO BENEDITO DOS SANTOS, IAPAR/UEPR; ROGÉRIO BARBOSA DE LIMA, UFPR; GETÚLIO TAKASHI NAGASHIMA, IAPAR; CARMEN LUCIA DE OLIVEIRA PETOKOWICZ, UFPR; VALÉRIA CARPENTIERI-PÍPOLO, Universidade Estadual de Londrina; LUIZ FILIPE PROTASIO PEREIRA, SAPC; DOUGLAS SILVA DOMINGUES, IAPAR; LUIZ GONZAGA ESTEVES VIEIRA, Universidade do Oeste Paulista.
Ano de publicação:	2015
Referência:	Genetics and Molecular Biology, v. 38, n. 2, p. 182-190, 2015
Conteúdo:	ncreased synthesis of galactinol and raffinose family oligosaccharides (RFOs) has been reported in vegetative tissues in response to a range of abiotic stresses. In this work, we evaluated the transcriptional profile of a Coffea canephora galactinol synthase gene (CcGolS1) in two clones that differed in tolerance to water deficit in order to assess the contribution of this gene to drought tolerance. The expression of CcGolS1 in leaves was differentially regulated by water deficit, depending on the intensity of stress and the genotype. In clone 109A (drought-susceptible), the abundance of CcGolS1 transcripts decreased upon exposure to drought, reaching minimum values during recovery from severe water deficit and stress. In contrast, CcGolS1 gene expression in clone 14 (drought-tolerant) was stimulated by water deficit. Changes in galactinol and RFO content did not correlate with variation in the steady-state transcript level. However, the magnitude of increase in RFO accumulation was higher in the tolerant cultivar, mainly under severe water deficit. The finding that the drought-tolerant coffee clone showed enhanced accumulation of CcGolS1 transcripts and RFOs under water deficit suggests the possibility of using this gene to improve drought tolerance in this important crop.
Thesagro:	Café
NAL Thesaurus:	Water stress
Palavras-chave:	Raffinose family oligosaccharides (RFOs) Coffee
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (SAPC)