Título:	The recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora.
Autoria:	LACERDA, A. F. SARTO, R. P. del SILVA, M. S. VASCONCELOS, E. A. R. de COELHO, R. R. SANTOS, V. O. dos GODOY, C. V. SEIXAS, C. D. S. SILVA, M. C. M. da GROSSI-DE-SA, M. F.
Afiliação:	ARIANE FERREIRA LACERDA, Universidade Federal do Rio Grande do Norte; RAFAEL PERSEGHINI DEL SARTO, Centro Universitário do Distrito Federal; MARILIA SANTOS SILVA, Cenargen; ERICO AUGUSTO ROSAS DE VASCONCELOS; ROBERTA RAMOS COELHO; VANESSA OLINTO DOS SANTOS, Universidade Federal do Rio Grande do Norte; CLAUDIA VIEIRA GODOY, CNPSO; CLAUDINE DINALI SANTOS SEIXAS, CNPSO; MARIA CRISTINA MATTAR DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen.
Ano de publicação:	2016
Referência:	3 Biotech, v. 6, n. 1, p. 59, Feb. 2016.
Conteúdo:	Plant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases.
Thesagro:	Soja Algodão Doença de planta Fungo
NAL Thesaurus:	Soybeans Cotton Plant diseases and disorders Fungi
ISSN:	2190-5738
Digital Object Identifier:	10.1007/s13205-015-0320-7
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPSO)