Título:	Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie.
Autoria:	GONÇALVES, A. N. D. SOARES, C. O. SANCHES, S. C. REIS, F. A. ROSINHA, G. M. S.
Afiliação:	ALINE NAJARA DOMINGOS GONÇALVES, Universidade Federal de Mato Grosso do sul; CLEBER OLIVEIRA SOARES, CNPGC; SIMONE CAMARGO SANCHES, Universidade Federal de Mato Grosso do Sul; FERNANDO ALVARENGA REIS, CNPC; GRACIA MARIA SOARES ROSINHA, CNPGC.
Ano de publicação:	2016
Referência:	Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.684-687, maio, 2016.
Conteúdo:	The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.
NAL Thesaurus:	genotyping prion diseases
Palavras-chave:	Encefalopatia espongiforme transmissivel Gene PRNP Genotipagem Polimorfismo de nucleotídeo único Single nucleotide polymorphisms Transmissible spongiform encephalopathies
Notas:	Notas científicas.
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPGC)