Título:	Solid cation exchange phase to remove interfering anthocyanins in the analysis of other bioactive phenols in red wine.
Autoria:	SILVA, L. F. da GUERRA, C. C. KLEIN, D. BERGOLD, A. M.
Afiliação:	LETICIA FLORES DA SILVA, CNPUV; CELITO CRIVELLARO GUERRA, CNPUV; Diandra Klein, Faculdade de Farmácia, Universidade de Caxias do Sul; Ana Maria Bergold, Laboratório de Química Farmacêutica, Universidade Federal do Rio Grande do Sul.
Ano de publicação:	2017
Referência:	Food Chemistryl, v. 227, p. 158-165, 2017.
Conteúdo:	Bioactive phenols (BPs) are often targets in red wine analysis. However, other compounds interfere in the liquid chromatography methods used for this analysis. Here, purification procedures were tested to eliminate anthocyanin interference during the determination of 19 red-wine BPs. Liquid chromatography, coupled to a diode array detector (HPLC-DAD) and a mass spectrometer (UPLC-MS), was used to compare the direct injection of the samples with solid-phase extractions: reversed-phase (C18) and strong cation-exchange (SCX). The HPLC-DAD method revealed that, out of 13 BPs, only six are selectively analyzed with or without C18 treatment, whereas SCX enabled the detection of all BPs. The recovery with SCX was above 86.6% for eight BPs. Moreover, UPLC-MS demonstrated the potential of SCX sample preparation for the determination of 19 BPs. The developed procedure may be extended to the analysis of other red wine molecules or to other analytical methods where anthocyanins may interfere. Chemical compounds studied in this article trans-Resveratrol (PubChemCID: 445154); trans-?-Viniferin (PubChemCID: 5315233); Quercetin (PubChemCID: 5280343); Myricetin (PubChemCID: 5281672); Kaempferol (PubChemCID: 5280863); trans-Cinnamic acid (PubChemCID: 444539); trans-Piceid (PubChemCID: 5281718); Taxifolin (PubChemCID: 439533) Keywords Bioactive phenols; Red wine; Liquid chromatography; Mass spectrometry; Diode array detector; Sample purification
NAL Thesaurus:	Liquid Chromatography
Palavras-chave:	Sample purification Bioactive phenols Diode array detector Mass spectometry Red wine
Digital Object Identifier:	http://dx.doi.org/10.1016/j.foodchem.2017.01.087
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPUV)