Title:	Cryopreservation of Coffea arabica L. zygotic embryos by vitrification.
Authors:	FREITAS, R. T. da PAIVA, R. SALES, T. S. SILVA, D. P. C. da REIS, M. V. dos SOUZA, A. C. de ROSA, S. D. V. F. da
Affiliation:	RODRIGO THEREZAN DE FREITAS, UFLA; RENATO PAIVA, UFLA; THAIS SILVA SALES, UFLA; DIOGO PEDROSA CORRÊA DA SILVA, UFLA; MICHELE VALQUÍRIA DOS REIS, UFLA; ANA CRISTINA DE SOUZA, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC.
Date Issued:	2016
Citation:	Notulac Botanicae Horti Agrobotanici Cluj-Napoca, v. 44, n. 2, p. 445-451, Dec. 2016.
Description:	As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.
NAL Thesaurus:	Ex situ conservation Histology Thawing
Keywords:	Liquid nitrogen PVS2 TTC test
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (SAPC)