Title:	Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids.
Authors:	RIBEIRO, C. A. G. PINTO, M. de O. MACIEL, T. E. F. PASTINA, M. M. GOMES, E. G. de GUIMARAES, C. T.
Affiliation:	Carlos Alexandre Gomes Ribeiro, Universidade Federal de Viçosa; MARCOS DE OLIVEIRA PINTO, CNPMS; Talles Eduardo Ferreira Maciel, Universidade Federal de Viçosa; MARIA MARTA PASTINA, CNPMS; Everaldo Gonçalves de Barros, Universidade Católica de Brasília; CLAUDIA TEIXEIRA GUIMARAES, CNPMS.
Date Issued:	2017
Citation:	Scientia Agricola, Piracicaba, v. 74, n. 2, p. 163-168, 2017.
Description:	The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.
Thesagro:	Zea mays Variedade
Keywords:	Genotipagem Caracterização genética
DOI:	10.1590/1678-992x-2016-0079
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMS)