Title:	Exploratory studies for cryopreservation of Coffea arabica L. seeds.
Authors:	FIGUEIREDO, M. A. de COELHO, S. V. B. ROSA, S. D. V. F. da VILELA, A. L. SILVA, L. C.
Affiliation:	MADELEINE ALVES DE FIGUEIREDO, UNIVERSIDADE FEDERAL DE LAVRAS STEFANIA VILAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS STTELA DELLYZETE VEIGA F DA ROSA, SAPC AMANDA LIMA VILELA, UNIVERSIDADE FEDERAL DE LAVRAS LUCIANO COUTINHO SILVA, UNIVERSIDADE FEDERAL DA PARAÍBA.
Date Issued:	2017
Citation:	Journal of Seed Science, v. 39, n. 2, p. 150-158, 2017.
Description:	Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.
Thesagro:	Coffea Arábica Resfriamento Conservação Germoplasma
NAL Thesaurus:	Water content silica gel Cryopreservation
DOI:	https://doi.org/10.1590/2317-1545v39n2171123
Notes:	Título em português: Estudos exploratórios para a criopreservação de sementes de Coffea arabica L.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (SAPC)