Title:	Taxonomic assessment and antimicrobial screening of isolated bacteria from marine and terrestrial Antarctic samples.
Authors:	SILVA, T. R. DUARTE, A. W. F. PASSARINI, M. R. Z. RUIZ, A. L. T. G. FRANCO, C. H. MORAES, C. B. FANTINATTI-GARBOGGINI, F. MELO, I. S. de RODRIGUES, R. A. OLIVEIRA, V. M.
Affiliation:	Tiago R. Silva, IB/UNICAMP Divisão de Resursos Microbianos, CPQBA UNICAMP Alysson W. F. Duarte, UFAL Alysson W. F. Duarte3, UNILA Ana Lucia T. G. Ruiz, Divisão de Farmacologia e Toxicologia, CPQBA UNICAMP Caio Haddad Franco, Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas Carolina Borsoi Moraes, Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas Fabiana Fantinatti-Garboggini1, UFAL ITAMAR SOARES DE MELO, CNPMA Rodney A. Rodrigues, Divisão de Química de Produtos Naturais, CPQBA UNICAMP Valéria M. de Oliveira.
Date Issued:	2018
Citation:	Revista Ciência, Tecnologia & Ambiente, Araras, SP, v. 6, n. 1, p. 542, 2017. Volume Especial. Edição dos Anais do VIII Simpósio de Microbiologia Aplicada, Rio Claro, maio 2017.
Description:	Microorganisms dominate most of Antarctic ecosystem and play a crucial role in its functioning. They are called extremophilic microorganisms with unique and versatile metabolic properties with possible biotechnological applications in several areas. The aim of the present study was to taxonomically characterize psychrophilic microorganisms from Antarctic continent samples and to screen them for antimicrobial effects. Phylogenetic analysis revealed that most isolates shared 98?100 % sequence similarity to recognized species, including those recovered previously from the Antarctica environment, which belong to the major phyla Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria (classes Alpha and Gammaproteobacteria). A total of 361 bacteria strains, distributed in 38 different genera, were recovered and identified based on sequencing of the 16S rRNA gene. The main representative genera were Arthrobacter (29%), Psychrobacter (19%), Pseudoalteromonas (10%) and Rhodococcus (4%). Antimicrobial screening of 600 bacteria revealed sixteen strains capable of inhibiting growth of at least one of the strains: E.coli, Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis and Candida albicans. One psychrotolerant bacterium, Pseudomonas mandelii strain 99, showed a broad antimicrobial range and was selected for further antiproliferative and antiparasitic tests due to low values in Minimal Inhibitory Concentration assay. Results demonstrated that the extremophiles from Antarctica represent an untapped source of diverse microorganisms capable of antimicrobial metabolite production.
NAL Thesaurus:	Antarctica
Keywords:	Antimicrobial activity Antiproliferative effect 16S rRNA genes Bacterial taxonomy
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CNPMA)