Title:	Diversity of Staphylococcus coagulase: positive and negative strains of coalho cheese and detection of enterotoxin encoding genes.
Authors:	ANDRADE, A. P. C. DE BORGES, M. DE F. EVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO ARCURI, E. F.
Affiliation:	ANA PAULA COLARES DE ANDRADE, Universidade Federal do Ceará, Departamento de Engenharia de Alimentos; MARIA DE FÁTIMA BORGES, Embrapa Agroindústria Tropical, Doutora, Pesquisadora aposentada.; EVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO, Universidade Federal do Ceará, Departamento de Engenharia de Alimentos; EDNA FROEDER ARCURI, CNPGL.
Date Issued:	2019
Citation:	Boletim Centro de Pesquisa de Processamento de Alimentos, Curitiba, v. 36, n. 1, p. 1-9, jan./jun. 2019.
Description:	Three hundred samples of coalho cheese, from 15 diff erent brands, seven handmade and eight industrialized, were evaluated in relation to the contamination profi le by Staphylococcus coagulase-positive and negative and the occurrence of staphylococcal enterotoxin encoding genes. Two hundred and eight isolates of Staphylococcus sp. were subjected to phenotypic identifi cation and 95 were subjected to genotypic identifi cation through femA gene research and detection of genes (sea, seb, sec, sed, see, seg, seh, sei and sej) encoding enterotoxins, using the polymerase chain reaction technique (PCR). A total of 14 species of Staphylococcus were identifi ed, of which three were coagulase-positive and eleven negative, especially: S. aureus, S. xylosus, S. cohnni spp. cohnii, S. saprophyticus, S. epidermidis, S. hyicus, S. lentus, S. sciuri, S. cohnii spp. urealyticus, S. haemolyticus, S. chromogenes, S. lugdunensis, S. hominis e S. intermedius. In all the samples of handmade coalho cheese there was a prevalence of S. aureus; while at industrial samples S. xylosus (87.5%) and S. cohnii spp cohnii (50%) were predominant. The presence of the femA gene was detected in 95% (38/40) isolates of positive Staphylococcus coagulase and 16.4% (9/55) of coagulasenegative isolates. Among the enterotoxin encoding genes evaluated, there was prevalence of the seh gene (53.2%) in coagulase-positive strains and of the sec gene (46.8%) in coagulase-negative strains. The results suggest a re-evaluation of the Brazilian microbiological standards in relation to the genus Staphylococcus in foods.
Thesagro:	Intoxicação Alimentar
NAL Thesaurus:	Dairy products Enterotoxins Staphylococcal intoxication
Keywords:	ENVENENAMENTO ALIMENTAR LACTICÍNIOS ENTEROTOXINAS ESTAFILOCÓCICAS
DOI:	https://doi.org/10.5380/bceppa.v36i1.57553
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)