Title:	Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli.
Authors:	SANTOS, M. R. A. dos PAZ, E. S.
Affiliation:	MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; Universidade Federal de Rondonia.
Date Issued:	2020
Citation:	International Journal of Development Research, v. 10, n. 11, p. 41853-41856, Nov. 2020.
Description:	In vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combinations. The percentage of callus induction (%CI), the explant area covered by callus cells (ACCC) and the fresh weight of the calluses were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The treatment that resulted in the highest %CI, ACCC and callus weight was 1.5 mg L-1 2,4-D. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 39th day of cultivation.
Thesagro:	Cultura In Vitro Calogênese Curva de Crescimento
NAL Thesaurus:	In vitro culture Callus formation Secondary metabolites Cell dedifferentiation
Keywords:	Capsicum frutescens cv Stromboli Célula foliar Metabólicos secundários Protocolo de diferenciação celular Leaf cells Callogenesis Growth curves Deceleration phase Fase de desaceleração Embrapa Rondônia Porto Velho (RO) Rondônia Amazônia Ocidental Western Amazon
ISSN:	2230-9926
DOI:	10.37118/ijdr.20362.11.2020
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAF-RO)