Title:	Priming of defense-related genes in Brassica oleracea var. capitata using concentrated metabolites produced by Rhizobium tropici CIAT 899.
Authors:	SANTOS, I. R. RIBEIRO, D. G. TÁVORA, F. T. P. K. MAXIMIANO, M. R. RABELO, A. C. RIOS, T. B. REIS JUNIOR, F. B. dos MEGÍAS, M. SILVA, L. P. da REIS, A. M. dos
Affiliation:	IVONALDO REIS SANTOS, UNIVERSIDADE DE BRASÍLIA; DAIANE GONZAGA RIBEIRO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; FABIANO TOUZDJIAN PINHEIRO KOHLRAUSCH TÁVORA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; MARIANA ROCHA MAXIMIANO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANA CAROLINA RABELO, UNIVERSIDADE DE BRASÍLIA; THUANNY BORBA RIOS, UNIVERSIDADE DE BRASÍLIA; FABIO BUENO DOS REIS JUNIOR, CPAC; MANUEL MEGÍAS, UNIVERSIDAD DE SEVILLA, SPAIN; LUCIANO PAULINO DA SILVA, CENARGEN; ANGELA MEHTA DOS REIS, CENARGEN.
Date Issued:	2022
Citation:	Brazilian Journal of Microbiology, v. 53, n. 2, 2022.
Description:	To verify the potential of metabolites extracted from Rhizobium tropici to trigger the priming of defense responses in cruciferous plants, we analyzed the expression of defense-related genes by qRT-PCR. Brassica oleracea var. capitata, susceptible to Xanthomonas campestris pv. campestris, were grown in greenhouse conditions. At 18 days after sowing, plants were inoculated with 1 mL of 1% concentrated metabolites produced by R. tropici (CM-RT) in the root. In a second experiment, leaves were sprayed with 1 mL of a solution containing 1% CM-RT. Aerial and root tissue were collected separately at 0 (non-treated control condition), 24, and 48 h after application, submitted to RNA extraction and gene expression analysis by qRT-PCR. The results showed that, after root treatment with CM-RT, most evaluated genes were upregulated at 24 h after application and downregulated at 48 h after application in roots, while in leaves, genes were downregulated both at 24 and 48 h after application. On the other hand, leaf treatment with CM-RT showed that most evaluated genes in leaves and roots were upregulated at 24 and 48 h after application. These results indicate that the effect of CM-RT applied in roots seems restricted to the applied region and is not sustained, while the application in leaves results in a more systemic response and maintenance of the effect of CM-RT for a longer period. The results obtained in this study emphasize the biotechnological potential of using metabolites of R. tropici as an elicitor of active defense responses in plants.
NAL Thesaurus:	Xanthomonas campestris pv. campestris
Keywords:	Defense response Concentrated metabolites
DOI:	https://doi.org/10.1007/s42770-022-00722-4
Notes:	Na publicação: Fábio Bueno Reis Junior, Luciano Paulino Silva, Angela Mehta.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAC)