Title:	Optimization of somatic embryogenesis in Coffea canephora.
Authors:	SILVA, C. K. C. da SANTOS, M. R. A. dos
Affiliation:	CAMILA KAREN CANDEIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.
Date Issued:	2022
Citation:	International Journal of Development Research, v. 12, n. 10, p. 59738-59740, Oct. 2022.
Description:	Plant tissue culture allows the clonal propagation of Coffea canephora on a large scale through somatic embryogenesis. The aim of this study was to optimize the conditions for the induction of somatic embryogenesisin leaves of the Robusta variety. The physiological stage of the leaves - drain or source; the use of the surrounding area ofprimary or secondary veins; and the position of the explants - abaxial or adaxial surface in contact with the culture medium were evaluated. The explants were inoculated in a modified MS culture medium (1/2 macro, 1/4 micronutrients), supplemented with 30 g L-1 sucrose, 6 g L-1 agar, 1.0 mg L-1 2iP and 5.0 mg L-1 IBA. At 45 days of culture, the occurrence of callus induction and the dry weight of the explants were evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The leaf fragments most responsive to the induction of somatic embryogenesis were taken from drain leaves, with explants containing secondary veins, placed with the adaxial face in contact with the culture medium.
Thesagro:	Café Robusta Coffea Canephora Cultura de Tecido Campo Experimental
NAL Thesaurus:	Tissue culture Somatic embryogenesis Demonstration farms
Keywords:	Cotyledonary embryos Embriogênese somática Embrapa Rondônia Porto Velho (RO) Amazônia Ocidental Western Amazon
ISSN:	2230-9926
DOI:	https://doi.org/10.37118/ijdr.25621.10.2022
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAF-RO)