Título:	Population genomics provide insights into the global genetic structure of Colletotrichum graminicola, the causal agent of maize anthracnose.
Autoria:	ROGÉRIO, F. BARONCELLI, R. CUEVAS-FERNÁNDEZ, F. B. BECERRA, S. CROUCH, J. BETTIOL, W. AZCÁRATE-PERIL, M. A. MALAPI-WIGHT, M. ORTEGA, V. BETRAN, J. TENUTA, A. DAMBOLENA, J. S. ESKER, P. D. REVILLA, P. JACKSON-ZIEMS HILTBRUNNER, J. MUNKVOLD, G. BUHINICEK, I. VICENTE-VILLARDÓN, J. L.'''' SUKNO, S. A. THON, M. R.
Afiliação:	FLÁVIA ROGÉRIO, UNIVERSIDAD DE SALAMANCA; RICCARDO BARONCELLI, UNIVERSITÀ DI BOLOGNA; FRANCISCO BORJA CUEVAS-FERNÁNDEZ, UNIVERSIDAD DE SALAMANCA; SIOLY BECERRA, UNIVERSIDAD DE SALAMANCA; JOANNE CROUCH, UNITED STATES DEPARTMENT OF AGRICULTURE; WAGNER BETTIOL, CNPMA; MARIA ANDREA AZCÁRATE-PERIL, UNIVERSITY OF NORTH CAROLINE; MARTHA MALAPI-WIGHT, UNITED STATES DEPARTMENT OF AGRICULTURE; VERONIQUE ORTEGA, SYNGENTA SEEDS; JAVIER BETRAN, BAYER CROP SCIENCE; ALBERT TENUTA, UNIVERSITY OF GUELPH-RIDGETOWN; JOSÉ S DAMBOLENA, UNIVERSIDAD NACIONAL DE CÓRDOBA; PAUL D ESKER, THE PENNSYLVANIA STATE UNIVERSITY; PEDRO REVILLA, CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS; TAMRA A JACKSON-ZIEMS, UNIVERSITY OF NEBRASKA-LINCOLN; JÜRG HILTBRUNNER, DÉPARTEMENT FÉDÉRAL DE L'ÉCONOMIE, DE LA FORMATION ET DE LA RECHERCHE; GARY MUNKVOLD, IOWA STATE UNIVERSITY; IVICA BUHINICEK, BC INSTITUTE FOR BREEDING AND PRODUCTION OF FIELD CROPS; JOSÉ LUIS VICENTE-VILLARDÓN, UNIVERSIDAD DE SALAMANCA; SERENELLA A SUKNO, UNIVERSIDAD DE SALAMANCA; MICHAEL R THON, UNIVERSIDAD DE SALAMANCA.
Ano de publicação:	2022
Referência:	MBio, v. 14, n. 1, e0287822, 2022.
Conteúdo:	Abstract: Understanding the genetic diversity and mechanisms underlying genetic variation in pathogen populations is crucial to the development of effective control strategies. We investigated the genetic diversity and reproductive biology of Colletotrichum graminicola isolates which infect maize by sequencing the genomes of 108 isolates collected from 14 countries using restriction site-associated DNA sequencing (RAD-seq) and whole-genome sequencing (WGS). Clustering analyses based on single-nucleotide polymorphisms revealed three genetic groups delimited by continental origin, compatible with short-dispersal of the pathogen and geographic subdivision. Intra- and intercontinental migration was observed between Europe and South America, likely associated with the movement of contaminated germplasm. Low clonality, evidence of genetic recombination, and high phenotypic diversity were detected. We show evidence that, although it is rare (possibly due to losses of sexual reproduction- and meiosis-associated genes) C. graminicola can undergo sexual recombination. Our results support the hypotheses that intra- and intercontinental pathogen migration and genetic recombination have great impacts on the C. graminicola population structure. Importance: Plant pathogens cause significant reductions in yield and crop quality and cause enormous economic losses worldwide. Reducing these losses provides an obvious strategy to increase food production without further degrading natural ecosystems; however, this requires knowledge of the biology and evolution of the pathogens in agroecosystems. We employed a population genomics approach to investigate the genetic diversity and reproductive biology of the maize anthracnose pathogen (Colletotrichum graminicola) in 14 countries. We found that the populations are correlated with their geographical origin and that migration between countries is ongoing, possibly caused by the movement of infected plant material. This result has direct implications for disease management because migration can cause the movement of more virulent and/or fungicide-resistant genotypes. We conclude that genetic recombination is frequent (in contrast to the traditional view of C. graminicola being mainly asexual), which strongly impacts control measures and breeding programs aimed at controlling this disease.
Thesagro:	Milho Antracnose Colletotrichum Graminicola Variação Genética
NAL Thesaurus:	Fungal diseases of plants Anthracnose Corn Population genetics Phylogeography Genetic recombination Genomics
ISSN:	2150-7511
Digital Object Identifier:	https://doi.org/10.1128/mbio.02878-22
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMA)