Title:	Cytotaxonomy and karyotype evolution in Neotropical Meliponini (Hymenoptera: Apidae) inferred by chromosomal mapping of 18S rDNA and five microsatellites.
Authors:	CUNHA, M. S. GARCIA, M. V. B. CAMPOS, L. A. O. LOPES, D. M.
Affiliation:	MARINA SOUZA CUNHA, UNIVERSIDADE FEDERAL DE VIÇOSA; MARCOS VINICIUS BASTOS GARCIA, CPAA; LUCIO ANTONIO OLIVEIRA CAMPOS, UNIVERSIDADE FEDERAL DE VIÇOSA; DENILCE MENESES LOPES, UNIVERSIDADE FEDERAL DE VIÇOSA.
Date Issued:	2023
Citation:	Journal of Apicultural Research, 2023.
Description:	The Neotropical Meliponini bees, commonly known as stingless bees, are phylogenetically subdivided into three clades in which the chromosome numbers vary from n 1⁄4 8 to n 1⁄4 17. The goal of this study was to identify the major chromosomal rearrangements that occurred during the Neotropical Meliponini (Apidae) karyotypic evolution. In this way, we mapped 18S rDNA and five microsatellites in 33 stingless bee species collected from different Brazilian regions. The species belonged to 15 genera and showed six different chromosome numbers: n 1⁄4 8, n 1⁄4 9, n 1⁄4 11, n 1⁄4 14, n 1⁄4 15, and n 1⁄4 17. The 18S rDNA probe showed a variation from 2 to 12 marked chromosomes in different positions (terminal, subterminal, or centromeric), including 2 B chromosomes out of the 7 B found in Tetragonisca fiebrigi. The microsatellite (GA)15, (GAG)10, (CAA)10, and (TCAGG)6 probes formed clusters on the euchromatic regions of the chromosomes and were useful in the identification of putative Robertsonian fusion events that led to the decrease in the chromosome number during the evolution of the Neotropical Meliponini clade. (TTAGG)6 constituted the telomeric sequence of the analyzed species. The ancestral state of the three Neotropical Meliponini clades is difficult to infer, although, the putative ancestral karyotype probably had a single pair of 18S rDNA cistrons, and the decrease in chromosome number and increase in the 18S rDNA sites occurred independently between genera.
NAL Thesaurus:	Stingless bees
Keywords:	Chromosome evolution Cytotaxonomy Microsatellites Molecular cytogenetics
DOI:	10.1080/00218839.2023.2179228
Notes:	Online first.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAA)