Title:	Assessment of the virulence of the Burkholderia mallei Strain BAC 86/19 in BALB/c Mice.
Authors:	GASPAR, E. B. SANTOS, L. R. dos EGITO, A. A. do SANTOS, M. G. dos MANTOVANI, C. RIEGER, J. DA S. G. ABRANTES, G. A. DE S. SUNIGA, P. A. P. FAVACHO, J. DE M. PINTO, I. B. NASSAR, A. F. DE C. SANTOS, F. L. DOS ARAUJO, F. R.
Affiliation:	EMANUELLE BALDO GASPAR, CPPSUL; LENITA RAMIRES DOS SANTOS, CNPGC; ANDREA ALVES DO EGITO, CNPGC; MARIA GORETTI DOS SANTOS, CNPGC; CYNTHIA MANTOVANI, CNPGC; JULIANA DA SILVA GOMES RIEGER, CNPGC; GUILHERME AUGUSTO DE SOUSA ABRANTES, CNPGC; PAULA ADAS PEREIRA SUNIGA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; JÚLIA DE MENDONÇA FAVACHO, CNPGC; INGRID BATISTA PINTO, CNPGC; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR, CENTER FOR RESEARCH AND DEVELOPMENT IN ANIMAL HEALTH; FERNANDO LEANDRO DOS SANTOS, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; FLABIO RIBEIRO DE ARAUJO, CNPGC.
Date Issued:	2023
Citation:	Microorganisms, v. 11, issue 10, e2597, 2023.
Description:	ABSTRACT - Burkholderia mallei is an aerobic, Gram-negative, non-motile bacillus. As an obligate mammalian pathogen, it primarily affects solipeds. Although rarely transmitted to humans, the disease it causes, glanders, is classified as a zoonosis. The bacterium was officially eradicated in Brazil in 1969; however, it reemerged after three decades. This study aims to assess the virulence of a specific B. mallei strain, isolated in Brazil, in BALB/c mice through intranasal infection. The strain, B. mallei BAC 86/19, was obtained from the tracheal secretion of a young mare displaying positive serology but no clinical signs of glanders. Post-mortem examinations revealed macroscopic lesions consistent with the disease, however. In mice, the LD50 was determined to be approximately 1.59 × 105 colony-forming units (CFU)/animal. Mice exposed to either 0.1 × LD50 or 1 × LD50 displayed transient weight loss, which resolved after three or five days, respectively. B. mallei persisted within the liver and lung for five days post-infection and in the spleen for seven days. These findings underscore the detectable virulence of the Brazilian B. mallei BAC 86/19 strain in mice, which are relatively resilient hosts. This research points to the importance of the continued investigation of the virulence mechanisms and potential countermeasures associated with B. mallei infections, including their Brazilian isolates.
Thesagro:	Doença Animal Zoonose
NAL Thesaurus:	Animal diseases Animal models Burkholderia mallei Glanders Pathogenicity Virulence Zoonoses
DOI:	https://doi.org/10.3390/microorganisms11102597
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGC)