Title:	Effects of natural antimicrobial compounds propolis and copaiba on periodontal ligament fibroblasts, molecular docking, and in vivo study in Galleria mellonella.
Authors:	PEDRINHA, V. F. SANTOS, L. M. GONÇALVES, C. P. GARCIA, M. T. LAMEIRA, O. A. QUEIROGA, C. L. MARCUCCI, M. C. SHAHBAZI, M.-A. SHARMA, P. K. JUNQUEIRA, J. C. SIPERT, C. R. ANDRADE, F. B. de
Affiliation:	VICTOR FELIZ PEDRINHA, UNIVERSIDADE DE SÃO PAULO; LETÍCIA MARTINS SANTOS, UNIVERSIDADE DE SÃO PAULO; CAROLINA PASSARELLI GONÇALVES, UNIVERSIDADE FEDERAL DO ABC; MAÍRA TERRA GARCIA, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; OSMAR ALVES LAMEIRA, CPATU; CARMEN LUCIA QUEIROGA, UNIVERSIDADE ESTADUAL DE CAMPINAS; MARIA CRISTINA MARCUCCI, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; MOHAMMAD-ALI SHAHBAZI, UNIVERSITY OF GRONINGEN; PRASHANT KUMAR SHARMA, UNIVERSITY OF GRONINGEN; JULIANA CAMPOS JUNQUEIRA, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; CARLA RENATA SIPERT, UNIVERSIDADE DE SÃO PAULO; FLAVIANA BOMBARDA DE ANDRADE, UNIVERSIDADE DE SÃO PAULO.
Date Issued:	2024
Citation:	Biomedicine & Pharmacotherapy, v. 171, 116139, 2024.
Description:	Root canal treatment addresses infectious processes that require control. Occasionally, the radicular pulp is vital and inflamed, presenting a superficial infection. To preserve pulpal remnants, conservative procedures have gained favor, employing anti-inflammatory medications. This study investigated the effects of propolis (PRO), and copaiba oil-resin (COR) associated with hydrocortisone (H) and compared their impact to that of Otosporin® concerning cytotoxic and genotoxic activity, cytokine detection, and toxicity in the Galleria mellonella model. Human periodontal ligament fibroblasts (PDLFs) were exposed to drug concentrations and evaluated by the MTT assay. Associations were tested from concentrations that did not compromise cell density. Genotoxicity was evaluated through micronucleus counting, while cytokines IL-6 and TGF-β1 were detected in the cell supernatant using ELISA. Molecular docking simulations were conducted, considering the major compounds identified in PRO, COR, and H. Increasing concentrations of PRO and COR were assessed for acute toxicity in Galleria mellonella model. Cellular assays were analyzed using one-way ANOVA followed by Tukey tests, while larval survivals were evaluated using the Log-rank (Mantel-Cox) test (α = 0.05). PRO and COR promoted PDLFs proliferation, even in conjunction with H. No changes in cell metabolism were observed concerning cytokine
Thesagro:	Própolis
NAL Thesaurus:	Toxicity
Keywords:	Toxicidade Doenças da polpa dentária Ancoragem molecular
DOI:	https://doi.org/10.1016/j.biopha.2024.116139
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATU)