Title:	Meloidogyne incognita control in cotton mediated host-induced gene silencing.
Authors:	SANTOS, M. M. dos SÁ, M. E. L. de LEMES, S. V. da R. CAVALCANTE, R. dos S. MORGANTE, C. V. SA, M. F. G. de
Affiliation:	MATEUS MEIRA DOS SANTOS, UNIVERSIDADE DE BRASILIA MARIA EUGÊNIA LISEI DE SÁ, EPAMIG SARA VITORINO DA ROCHA LEMES, UNIVERSIDADE DE BRASÍLIA RAÍRE DOS SANTOS CAVALCANTE, UNIVERSIDADE DE BRASILIA CAROLINA VIANNA MORGANTE, CPATSA MARIA FATIMA GROSSI DE SA, CENARGEN.
Date Issued:	2023
Citation:	In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023.
Pages:	p. 582.
Description:	In the present work we used a Host-Induced Gene Silencing (HIGS) strategy employing RNA interference (RNAi) mechanisms in order to silence RKN essential genes involved in development (cathepsin L cysteine proteinase - MiCPL), metabolism (isocitrate lyase - MiICL), mRNA processing (splicing factor - MiSF) and parasitism (nematode effector Mi16D-10). We staked all four RKN genes into a single construct (dsMINC2), driven by the pUceS8.3 constitutive soybean promoter, in order to control the nematode reproduction in transgenic cotton plants developed from an RKN-susceptible cultivar (BRS372). Transgenic dsMinc2-T2 plants infected with Meloidogyne incognita showed a significant reduction in the number of eggs/g.root -1 (73 - 93%) as well as in the reproduction factor (80-93%), compared with the susceptible non-transgenic cultivar.
Thesagro:	Algodão Gossypium Hirsutum Doença Planta Transgênica Produção Agrícola
NAL Thesaurus:	Small interfering RNA Root-knot nematodes Transgenic plants Cotton
Keywords:	Nematóide das galhas
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CPATSA)