Title:	The chromosome number and heterochromatin distribution pattern suggest differentiation among species of the Stylosanthes scabra-seabrana complex.
Authors:	LIRA, I. C. S. A. MELO, N. F. de ANTONIO, R. P. OLIVEIRA, R. S. de QUEIROZ, M. A.
Affiliation:	IRLANE CRISTINE S.A. LIRA, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA; NATONIEL FRANKLIN DE MELO, CPATSA; RAFAELA PRISCILA ANTONIO, CPATSA; RONALDO SIMÃO DE OLIVEIRA, INSTITUTO FEDERAL DE EDUCAÇÃO CIÊNCIAS E TECNOLOGIA BAIANO; MANOEL ABILIO DE QUEIROZ, UNIVERSIDADE DO ESTADO DA BAHIA.
Date Issued:	2024
Citation:	Anais da Academia Brasileira de Ciências, v. 96, e20240247, 2024.
Description:	The genus Stylosanthes has economic importance in semi-arid regions and requires studies that reveal complex relationships between species involving of Stylosanthes identified as S. scabra, in order to properly identify the number, morphology, and pattern of distribution of heterochromatin, analyzing the karyological variability of these species. Accessions with 2n=40 and 2n=20 were identified, exhibiting semi-reticulate interphase nuclei, symmetric karyotype, varied morphology, with differences in average chromosomal size, and genome length. The analysis with the fluorochromes chromomycin (CMA) and 4’,6-diamidino-2-phenylindole (DAPI) allowed the visualization of two CMA+blocks in the subterminal region of the hort arm in all accessions, and DAPI+ /CMAbands in S. scabra accessions. This suggests that not only chromosomal rearrangements, but also changes in the composition of heterochromatin, may have occurred during the speciation of this genus, and that S. scabra may be undergoing chromosomal evolution based on the observed karyological differences. In addition to the ploidy level, the distribution pattern of CMA+ heterochromatin reinforces the separation between S. scabra and S. seabrana. Thus, this genus represents an interesting group of plants for further studies on the content and quantity of repetitive and non-repetitive DNA sequences.
Thesagro:	Genética Cromossoma DNA Estilosante Planta Forrageira
NAL Thesaurus:	Staining Chromosomes Chromosome banding Genetic resources Genetic techniques and protocols Sequence diversity
Keywords:	Diversidade genética Bombeamento cromossômico Fluorocromos Sequências de DNA
ISSN:	1678-2690
DOI:	https://10.1590/0001-3765202420240247
Notes:	Suplemento 1.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)