Title:	An enzymatic and proteomic analysis of panus lecomtei during biodegradation of gossypol in cottonseed.
Authors:	SOARES NETO, C. B. GOMES, T. G. FERREIRA FILHO, E. X. FONTES, W. RICART, C. A. O. ALMEIDA, J. R. M. de SIQUEIRA, F. G. de MILLER, R. N. G.
Affiliation:	CLEMENTE BATISTA SOARES NETO, UNIVERSITY OF BRASÍLIA TAÍSA GODOY GOMES, UNIVERSITY OF BRASÍLIA EDIVALDO XIMENES FERREIRA FILHO, UNIVERSITY OF BRASÍLIA WAGNER FONTES, UNIVERSITY OF BRASÍLIA CARLOS ANDRÉ ORNELAS RICART, UNIVERSITY OF BRASÍLIA JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE FELIX GONCALVES DE SIQUEIRA, CNPAE ROBERT NEIL GERARD MILLER, UNIVERSITY OF BRASÍLIA.
Date Issued:	2024
Citation:	Journal of Fungi, v. 10, n. 5, 321, 2024.
Description:	Cotton is an important plant-based protein. Cottonseed cake, a byproduct of the biodiesel industry, offers potential in animal supplementation, although the presence of the antinutritional sesquiterpenoid gossypol limits utilization. The macrofungus Panus lecomtei offers potential in detoxification of antinutritional factors. Through an enzymatic and proteomic analysis of P. lecomtei strain BRM044603, grown on crushed whole cottonseed contrasting in the presence of free gossypol (FG), this study investigated FG biodegradation over a 15-day cultivation period. Fungal growth reduced FG to levels at 100 μg/g, with a complex adaptive response observed, involving primary metabolism and activation of oxidative enzymes for metabolism of xenobiotics. Increasing activity of secreted laccases correlated with a reduction in FG, with enzyme fractions degrading synthetic gossypol to trace levels. A total of 143 and 49 differentially abundant proteins were observed across the two contrasting growth conditions after 6 and 12 days of cultivation, respectively, revealing a dynamic protein profile during FG degradation, initially related to constitutive metabolism, then later associated with responses to oxidative stress. The findings advance our understanding of the mechanisms involved in gossypol degradation and highlight the potential of P. lecomtei BRM044603 in cotton waste biotreatment, relevant for animal supplementation, sustainable resource utilization, and bioremediation.
NAL Thesaurus:	Cotton Proteomics Biomass Gossypol Detoxification (processing) Animal feeding
Keywords:	Panus lecomtei
DOI:	https://doi.org/10.3390/jof10050321
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPAE)