Title:	Antisense oligonucleotide as a new technology application for CsLOB1 gene silencing aiming citrus canker resistance.
Authors:	LIMA, L. F. F. de CARVALHO, I. G. B. SOUZA-NETO, R. R. de SANTOS, L. da S. dos NASCIMENTO, C. A. TAKITA, M. A. TÁVORA, F. T. P. K. REIS, A. M. dos SOUZA, A. A. de
Affiliation:	LUIZ FELIPE FRANCO DE LIMA, AGRONOMIC INSTITUTE–IAC ISIS GABRIELA BARBOSA CARVALHO, ISIS GABRIELA BARBOSA CARVALHO REINALDO RODRIGUES DE SOUZA-NETO, AGRONOMIC INSTITUTE–IAC LARISSA DA SILVA DOS SANTOS, AGRONOMIC INSTITUTE–IAC CÉSAR AUGUSTO NASCIMENTO, AGRONOMIC INSTITUTE–IAC MARCO AURÉLIO TAKITA, AGRONOMIC INSTITUTE–IAC FABIANO TOUZDJIAN PINHEIRO KOHLRAUSCH TÁVORA ANGELA MEHTA DOS REIS, CENARGEN ALESSANDRA ALVES DE SOUZA, AGRONOMIC INSTITUTE–IAC.
Date Issued:	2024
Citation:	Phytopathology, v. 114, n. 8, 1802-1809, 2024.
Description:	Citrus canker disease, caused by Xanthomonas citri subsp. citri, poses a significant threat to global citrus production. The control of the disease in the field relies mainly on the use of conventional tools such as copper compounds, which are harmful to the environment and could lead to bacterial resistance. This scenario stresses the need for new and sustainable technologies to control phytopathogens, representing a key challenge in developing studies that translate basic into applied knowledge. During infection, X. citri subsp. citri secretes a transcriptional activator-like effector that enters the nucleus of plant cells, activating the expression of the canker susceptibility gene LATERAL ORGAN BOUNDARIES 1 (LOB1). In this study, we explored the use of antisense oligonucleotides (ASOs) with phosphorothioate modifications to transiently inhibit the gene expression of CsLOB1 in Citrus sinensis. We designed and validated three potential ASO sequences, which led to a significant reduction in disease symptoms compared with the control. The selected ASO3-CsLOB1 significantly decreased the expression level of CsLOB1 when delivered through two distinct delivery methods, and the reduction of the symptoms ranged from approximately 15 to 83%. Notably, plants treated with ASO3 did not exhibit an increase in symptom development over the evaluation period. This study highlights the efficacy of ASO technology, based on short oligonucleotide chemically modified sequences, as a promising tool for controlling phytopathogens without the need for genetic transformation or plant regeneration. Our results demonstrate the potential of ASOs as a biotechnological tool for the management of citrus canker disease.
Thesagro:	Xanthomonas Citri
Keywords:	Susceptibility gene
DOI:	https://doi.org/10.1094/PHYTO-02-24-0058-KC
Notes:	Na publicação: Angela Mehta.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CENARGEN)