Title:	Bioassay for detection of transgenic soybean seeds tolerant to glyphosate.
Authors:	TORRES, A. C. NASCIMENTO, W. M. PAIVA, S. A. V. ARAGÃO, F. A. S. de
Affiliation:	Antonio Carlos Torres, CNPH; Warley Marco Nascimento, CNPH; Sonia Alessandra Vasconcelos Paiva, CNPH; Fernando Antonio Souza de Aragão, CNPH.
Date Issued:	2003
Citation:	Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 9, p. 1053-1057, set. 2003
Description:	Glyphosate is a systemic, nonselective, postemergence herbicide that inhibits growth of both weeds and crop plants. Once inside the plant, glyphosate interferes with biosynthesis of aromatic amino acids phenylalanine, tyrosine, and tryptophan, by inhibiting the activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway. The objective of this work was to develop a simple, effective and inexpensible method for identification of transgenic soybean tolerant to glyphosate. This technique consisted in germinating soybean seeds in filter paper moistened with 100 to 200 mM of glyphosate. Transgenic soybean seeds tolerant to glyphosate germinated normally in this solution and, between 7 and 10 days, started to develop a primary root system. However non-transgenic seeds stopped primary root growth and emission of secondary roots.
Thesagro:	Biossíntese Glycine Max
NAL Thesaurus:	biosynthesis herbicide resistance transgenic plants
Keywords:	resistência a herbicida plantas transgênicas
Notes:	Título em português: Bioensaio para detecção de sementes de soja transgênicas tolerantes ao glifosato.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPH)