Title:	Role of Bacillus subtilis exopolymeric genes in modulating rhizosphere microbiome assembly.
Authors:	NISHISAKA, C. S. VENTURA, J. P. BAIS, H. P. MENDES, R.
Affiliation:	CAROLINE SAYURI NISHISAKA, UNIVERSIDADE DE SÃO PAULO; JOÃO PAULO VENTURA, UNIVERSIDADE DE SÃO PAULO; HARSH P BAIS, UNIVERSITY OF DELAWARE; RODRIGO MENDES, CNPMA.
Date Issued:	2024
Citation:	Environmental Microbiome, v. 19, article 33, 2024.
Description:	Abstract: Background: Bacillus subtilis is well known for promoting plant growth and reducing abiotic and biotic stresses. Mutant gene-defective models can be created to understand important traits associated with rhizosphere fitness. This study aimed to analyze the role of exopolymeric genes in modulating tomato rhizosphere microbiome assembly under a gradient of soil microbiome diversities using the B. subtilis wild-type strain UD1022 and its corresponding mutant strain UD1022eps−TasA, which is defective in exopolysaccharide (EPS) and TasA protein production. Results: qPCR revealed that the B. subtilis UD1022eps−TasA− strain has a diminished capacity to colonize tomato roots in soils with diluted microbial diversity. The analysis of bacterial β-diversity revealed significant differences in bacterial and fungal community structures following inoculation with either the wild-type or mutant B. subtilis strains. The Verrucomicrobiota, Patescibacteria, and Nitrospirota phyla were more enriched with the wild-type strain inoculation than with the mutant inoculation. Co-occurrence analysis revealed that when the mutant was inoculated in tomato, the rhizosphere microbial community exhibited a lower level of modularity, fewer nodes, and fewer communities compared to communities inoculated with wild-type B. subtilis.
Thesagro:	Bactéria Bactéria não Patogênica Rizosfera Tomate
NAL Thesaurus:	Bacillus subtilis Microbiome Tomatoes Rhizosphere bacteria Exopolysaccharides
Keywords:	PGPR TasA EPS Dilution to extinction
DOI:	https://doi.org/10.1186/s40793-024-00567-4
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMA)