Title:	Aeromonas hydrophila dose and post-immunostimulation period as immunomodulatory effect indicator on Nile tilapia (Oreochromis niloticus)
Authors:	OTA, E. do C. INOUE, L. A. K. A. ZANON, R. B. SUARES, F. S. SILVA, T. S. de C.
Affiliation:	ERIKA DO CARMO OTA, BOLSISTA / EMBRAPA AGROPECUÁRIA OESTE, DOURADOS; LUIS ANTONIO KIOSHI AOKI INOUE, CPAO; RICARDO BASSO ZANON, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS; FERNANDA SOTOLANI SUARES, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS; TARCILA SOUZA DE CASTRO SILVA, CPAO.
Date Issued:	2024
Citation:	Pesquisa Agropecuária Gaúcha, v. 30, n. 1, p. 80-99, 2024.
Description:	In fish challenge tests, there is no standardization in the concentration and analysis period of parameters. This study investigates the immunomodulatory and cytotoxic effects of A. hydrophila dose and post- immunostimulation periods on Nile tilapia. Four level of bacterial doses (1⁄2, 1⁄4, 1⁄8, and 0 LD50) were administered, and immune parameters, hematological data, and erythrocyte abnormalities were analyzed at 3, 7, 10, and 14 days post-infection (dpi). The highest respiratory burst, total globulins, and lysozyme activity were detected at 7 dpi. Considering the bacteria doses, the lysozyme activity was higher in the 1⁄8 LD50 and 1⁄4 LD50 of A. hydrophila. Erythrocytes, hematocrit, hemoglobin, leukocytes, lymphocytes, neutrophils, and monocytes counts remained unchanged. Ten days post-injection, the control group exhibited reduced abnormalities, showing a notable distinction from the 1⁄8 LD50 and 1⁄4 LD50 treatments, particularly in hooked and notched nuclei abnormalities. Mortality rates were higher in infected groups, peaking at different times with the highest in 1⁄2 LD50. For evaluating Nile tilapia immunity post A. hydrophila infection, it is recommended 1⁄4 LD50 dose and blood collection at 7 and 10 dpi for immunological parameters and abnormalities in Nile tilapia erythrocytes, respectively.
Keywords:	Tilapia do nilo Imunomoduladores Citotóxicos
DOI:	10.36812/pag.202430180-99
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAO)