Title:	Subtractive cDNA library and macro-array analysis to isolate specific genes related to the color diversity in the storage root of cassava (Manihot esculenta Crantz).
Authors:	SOUZA, C. R. B. de CARVALHO, L. J. C. B. CASCARDO, J. C. de M. AGOSTINI, M. A. V.
Affiliation:	CLAUDIA REGINA BATISTA DE SOUZA; LUIZ JOAQUIM CASTELO B CARVALHO, CENARGEN; JULIO CEZAR DE MATTOS CASCARDO; MARCO ANTONIO VALLE AGOSTINI.
Date Issued:	2004
Citation:	In: IINTERNATIONAL SCIENTIFIC MEETING CASSAVA BIOTECHOLOGY NETWORK, 6., 2004, Cali. Celebrating cassava diversity: a contribution from EMBRAPA/LBB. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. 1CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 111). Organized by Luiz J. C. B. Carvalho.
Description:	This study was performed to evaluate cDNA subtractive library in combination with macro-array technology to identify genes using their transcriptionally defective mutant in the diversity of pigmented storage root of cassava. Diversity of color in cassava storage root is due to the presence of a variety of carotenoids, including b-carotene, lycopene, phytoene and lutein that are synthesized and accumulated in chromoplast of this type of root. Three putative mutants in our GENEBANK were identified to accumulate solely lycopene, bcarotene, or lutein. If this differential accumulation is in the synthesis pathway or in the process of accumulation is not yet known. Three cDNA subtractive libraries were prepared and the generated ESTs are being tested for its tissue specificity, traditional clone specificity and for the tissue age effect in the storage root. Preliminary results indicate that the number of genes is differentially distributed among cellular and molecular processes including 20% coding for complex carbohydrate metabolism related genes, 16% coding for senescent cell process, 15% coding for protease as the major classes of genes. Secondary metabolism derived compounds genes code for 8% of the genes identified. Color phenotype and tissue age in the storage root are being tested for the differentially expressed EST using macro array and conventional mRNA blot as well as a set of eight genes coding for enzymes of the carotenoid syntheses pathway.
Keywords:	Manihot esculenta Crantz
Type of Material:	Artigo em anais e proceedings
Access:	openAccess
Appears in Collections:	Artigo em anais de congresso (CENARGEN)