Title:	Characterization of differentially expressed sequence tag (EST) from sugary phenotype of storage root of cassava (Manihot esculenta Crantz).
Authors:	CARVALHO, L. J. C. B. SOUZA, C. R. B. de CASCARDO, J. C. de M. AGOSTINI, M. A. V.
Affiliation:	LUIZ JOAQUIM CASTELO B CARVALHO, CENARGEN; CLAUDIA REGINA BATISTA DE SOUZA, UNIVERSIDADE FEDERAL DO PARÁ; JULIO CEZAR DE MATTOS CASCARDO, CELULARUNIVERSIDADE ESTADUAL DE SANTA CRUZ; MARCO ANTONIO VALLE AGOSTINI.
Date Issued:	2004
Citation:	In: IINTERNATIONAL SCIENTIFIC MEETING CASSAVA BIOTECHOLOGY NETWORK, 6., 2004, Cali. Celebrating cassava diversity: a contribution from EMBRAPA/LBB. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. 1CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 111). Organized by Luiz J. C. B. Carvalho.
Description:	The sugary storage root phenotype was, recently identified from traditional clones cultivated in Amazon. At the level of above ground morphology there is no particular distinction of sugary cassava from the farina and table cassava (see accompany poster). Major distinction among these types of cassava is observed only in the storage root, which shows profound alteration in storage root size and density in water, secondary xylem cells development, parenchyma cells size and turgidity, diverse starch structure presence, and osmotic parenchyma cells environment. Therefore it is expected to observe a profound effect on genes being expressed and regulated differentially in the sugary cassava in comparison with the farina type. We are using cDNA subtractive library to construct an EST database with silenced and expressed genes for this kind of storage root. Macro array and conventional mRNA blots are being used for differential gene expression analyzes. The BLAST and clustering analyses of 1200 high quality EST sequences gave 267 cDNA clones that were tested with macro array and conventional RNA blot analyzes. Preliminary gene sequence annotation for 953 cDNA clones indicated that 25% are non hits, 22% codes for unknown protein, 17% codes for carbohydrate related metabolism, and other minor functions. cDNA clones are being tested for gene expression analyzes to gain information on tissue specificity, tissue age effect as well as phenotype specificity.
Keywords:	Manihot esculenta Crantz
Type of Material:	Artigo em anais e proceedings
Access:	openAccess
Appears in Collections:	Artigo em anais de congresso (CENARGEN)