Title:	Comparative gene expression study to identify genes possibly related to storage root formation in cassava (Manihot Esculenta Crantz).
Authors:	SOUZA, C. R. B. de CARVALHO, L. J. C. B. CASCARDO, J. C. de M.
Affiliation:	CLÁUDIA REGINA BATISTA DE SOUZA, UNIVERSIDADE FEDERAL DO PARÁ; LUIZ JOAQUIM CASTELO B CARVALHO, CENARGEN; JULIO CESAR DE MATTOS CASCARDO, UNIVERSIDADE ESTADUAL DE SANTA CRUZ.
Date Issued:	2004
Citation:	In: IINTERNATIONAL SCIENTIFIC MEETING CASSAVA BIOTECHOLOGY NETWORK, 6., 2004, Cali. Celebrating cassava diversity: a contribution from EMBRAPA/LBB. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. 1CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 111). Organized by Luiz J. C. B. Carvalho.
Description:	Cassava storage roots result from swelling of adventitious roots by secondary growth. Storage root formation involves continuous elongation (primary growth) and radial growths. The cells of vascular cambium, a meristematic tissue, are rapidly divided and expanded resulting in secondary growth that gives an increased diameter, giving secondary phloem to the outside and secondary xylem to the inside with parenchymatic cells packed with starch. In the present work we aimed to gain insight into molecular processes occurring during cassava storage root formation. Identifying genes expressed during secondary growth, will be possible to elucidate molecular processes related to parenchyma cells development (starch, protein and carotenoid accumulations) and prospect specific promoter and enhancers able to drive efficient storage root specific expression. In this work, we report a comparative gene expression study by Northern blot analysis in adventitious and storage roots to identify such genes. Cassava genes clones from our cDNA library of storage root, available in the Cassava Databank at the Biochemistry and Biophysics of EMBRAPA Genetic Resources and Biotechnology, were sorted by molecular and cellular processes and used as probes in this study. Results, so far, revealed four classes expressed genes based on the intensity of the hybridization signal in the northern blot analysis.
Keywords:	Manihot Esculenta Crantz
Type of Material:	Artigo em anais e proceedings
Access:	openAccess
Appears in Collections:	Artigo em anais de congresso (CENARGEN)