Título:	Innate immunity trained in the protective response of vaccine candidates against intracellular pathogens.
Autoria:	OLIVEIRA, J. B. S. SILVA, L. A. SOUSA, M. F. S. FONSECA JUNIOR, A. M. ALMEIDA, C. G. BARDUCCI, R. S. MILAZZOTTO, M. P. BRANDAO, H. de M. SANTOS, R. L. PAIXÃO, T. A.
Afiliação:	JEFFERSON B. S. OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; LAICE A. SILVA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; MONIQUE F. S. SOUSA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ALDCEJAM MARTINS FONSECA JUNIOR, UNIVERSIDADE FEDERAL DO ABC; CAMILA G. ALMEIDA; ROBSON S. BARDUCCI, BIORIGIN; MARCELLA P. MILAZZOTTO, UNIVERSIDADE FEDERAL DO ABC; HUMBERTO DE MELLO BRANDAO, CNPGL; RENATO L. SANTOS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; TATIANE A. PAIXÃO, UNIVERSIDADE FEDERAL DE MINAS GERAIS.
Ano de publicação:	2026
Referência:	Vaccines, v. 14, 197026, 2026.
Conteúdo:	Background/Objectives: Trained innate immunity refers to the enhanced responsiveness of innate immune cells, particularly macrophages, following exposure to stimuli such as β-glucan or zymosan, enabling improved defense against unrelated pathogens. This phenomenon has been widely investigated to better understand host–pathogen interactions and to support the development of improved infection control strategies. This study evaluated whether these training stimuli could enhance the protective efficacy of attenuated or inactivated vaccine models against Brucella ovis and Listeria monocytogenes infection. Methods: Trained innate immunity was induced in vivo using β-glucan or zymosan, and seven days later mice were vaccinated with attenuated or gamma-irradiated formulations and subsequently challenged with B. ovis or L. monocytogenes. Vaccine-induced protection and immune responses were assessed through multiple experimental approaches. Results: β-glucan significantly reduced bacterial infection in vitro in bone-marrow-derived macrophages and in vivo in target organs compared with zymosan. Although β-glucan did not enhance the efficacy of the attenuated B. ovis ∆abcBA vaccine, it markedly reduced bacterial colonization in mice vaccinated with gamma-irradiated B. ovis. β-glucan also did not improve the efficacy of the gamma-irradiated L. monocytogenes vaccine; however, 50% of the trained and vaccinated mice showed no detectable bacterial recovery. Increasing the number of β-glucan doses negatively affected infection control, suggesting that overstimulation may impair trained immunity. Conclusion: Trained innate immunity enhances the protective effect of inactivated experimental vaccines against B. ovis and L. monocytogenes, while exerting a detrimental influence on the efficacy of a live attenuated B. ovis vaccine model.
Thesagro:	Bovino Doença Animal Patógeno Imunidade Brucelose Vacina Listeriose
Palavras-chave:	Zimosano
Digital Object Identifier:	https://doi.org/10.3390/vaccines14020197
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPGL)