Título:	Seed viability of Commiphora leptophloeos (Mart.) J.B. Gillett (Burseraceae) assessed by the Tetrazolium test.
Autoria:	SILVA, J. C. da SILVA, J. de J. GOMES, R. A. CRUZ, C. R. P. DANTAS, B. F.
Afiliação:	JAMILLE CARDEAL DA SILVA, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA JAILTON DE JESUS SILVA, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA RAQUEL ARUJO GOMES, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA CLAUDINÉIA REGINA PELANCANI CRUZ, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA BARBARA FRANCA DANTAS, CPATSA.
Ano de publicação:	2025
Referência:	International Journal of Plant Biology, v. 16, n. 4, 139, 2025.
Conteúdo:	Commiphora leptophloeos, a native Caatinga species with economic and medicinal potential, faces propagation challenges due to seed dormancy and extractive use. The germination test, the official method for seed quality assessment, is time-consuming, whereas the tetrazolium test (TZT) offers a rapid alternative for determining seed viability. This study aimed to establish and validate a TZT protocol for C. leptophloeos seeds. Seeds collected in 2025 were extracted after natural fruit drying and then stored in a cold chamber. The germination test was conducted with seeds without pyrenes at 30 ◦C. For the TZT, a completely randomized design was used in a 6 × 4 factorial scheme (six TZT concentrations × four immersion times), with adjustments in seed preparation and staining procedures. Higher concentrations (0.5% and 0.75%) combined with shorter immersion periods (2 h) provided the best results, especially 0.75% for 2 h, which yielded 89% viability. Very low concentrations combined with short periods resulted in little or no staining. Compared with the germination test (35%), the TZT showed greater sensitivity in detecting viable seeds. We conclude that the TZT is highly efficient for assessing the viability of C. leptophloeos seeds, with optimal responses at 0.5–0.75% TTC and 2–4 h immersion periods, and represents a strategic tool to support the conservation and sustainable use of this species.
Thesagro:	Espécie Nativa Caatinga Semente Planta Medicinal Teste de Tetrazólio
NAL Thesaurus:	Natural resources
Palavras-chave:	Commiphora leptophloeos Bioma Caatinga Imburana-de-espinho Umburana-de-cambão
Digital Object Identifier:	https://doi.org/10.3390/ijpb16040139
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)