Título:	Transferability of microsatellite and sequence tagged site markers in Oryza species.
Autoria:	BRONDANI, C. RANGEL, P. H. N. BORBA, T. C. O. BRONDANI, R. P. V.
Afiliação:	CLAUDIO BRONDANI, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
Ano de publicação:	2003
Referência:	Hereditas, v. 138, p. 187-192, 2003.
Conteúdo:	The genus Oryza comprises 22 species which are potentially useful as a source of genetic variability that can be introgressed into the worldwide cultivated rice, Oryza sativa. Molecular markers are useful tools for monitoring gene introgressions and for detecting polymorphism among species. In this study, cross-amplification was estimated among 28 accessions of 16 Oryza species, representing the genomes AA, BB, CC, BBCC and CCDD, using 59 microsatellite (OG, OS and RM series) and 15 STS (Sequence Tagged Sites) markers. All markers amplified at least one Oryza species, indicating different levels of transferability across species. Markers based on microsatellite sequences amplified 37 % of the accessions, with an average of 6.58 alleles per locus and an average polymorphism information content (PIC) of 70 %. For STS markers, the amplification level was 53.3 %, and the average number of alleles and PIC values were 1.6 and 10 %, respectively. These Results showed that although the STS markers detected a reduced level of genetic diversity, the transferability was higher, indicating that they can be used for genetic analysis when evaluating less genetically related species of Oryza. Among the microsatellite markers, an analysis of species with an AA genome showed that the OG markers produced the highest level of polymorphic loci (54.6 %), followed by RM markers (48 %). Highly polymorphic and transferable molecular markers in Oryza can be useful for exploiting the genetic resources of this genus, for detecting allelic variants in loci associated with important agronomic traits, and for monitoring alleles introgressed from wild relatives to cultivated rice.
Thesagro:	Arroz Oryza Sativa Marcador Molecular
NAL Thesaurus:	Rice Microsatellite repeats Genetic markers
Palavras-chave:	Microsatellite
Digital Object Identifier:	10.1034/j.1601-5223.2003.01656.x
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPAF)