Title:	Multicomutation flow system for spectrophometric total amino acids determination in plant material.
Authors:	NOGUEIRA, A. R. de A. OLIVEIRA, D. B. CARAPELI, R. FERRAZ, L. F. de M. MARTELLI, P. B.
Affiliation:	ANA RITA DE ARAUJO NOGUEIRA, CPPSE; DANIEL B. OLIVEIRA, UFSCar/SÃO CARLOS, SP.; RODOLFO CARAPELI, UFSCar/SÃO CARLOS, SP.; LUIZ FRANCISCO DE MATTEO FERRAZ, CNPDIA; PATRICIA B. MARTELLI, UNIVERSIDADE DE SÃO JOÃO DEL REI, MG.
Date Issued:	2006
Citation:	In: INTERNATIONAL CONFERENCE FLOW ANALYSIS, 10., 2006, Porto. Proceedings... Porto: IAFA, 2006
Pages:	p.183
Description:	To identify lhe nitrogen transportation forms and attain the control mechanism, the amount of free amino acids in different parts of lhe plant has to be determined. An automatic, fast and reliable procedure multicomutated flow system {1] has been developed for spectrophotometric analysis of totalamino acids in plant material. The method is suitable for routine analysis for a large number of samples of plant material. The flow manifold was designed with computer-controlled three-way solenoid valves for independent handling of sample and reagent solutions and a data acquisition system from a spectrophotometer, employed for signal measurements. The software for system control was performed bya program with use of a LabView platform INationallntruments) [2]. The detection reaction was based on the complexation of amino functional groups of amino acids by ninhydrin. It reacts with free a-amino groups, producing lhe colored ninhydrin chromophore called Ruhemann's purple IRP) IAm". = 570 nm; E = 22 000) [3]. The proposed detection system shows a linear range concentration up to 2.0 X 10-3 mal L-I with coefficient of variation of 1.1% (n = 101. Detection limits were estimated as 2.8 x 10-3 mal L-I at 99.7% confidence level for total amino acids. and a mean sampling rale of 30 determinations per hour was achieved.
Keywords:	Flow system Multicomutation
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CPPSE)