Título:	Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata.
Autoria:	JONSSON, C. M. AOYAMA, H.
Afiliação:	CLAUDIO MARTIN JONSSON, CNPMA; HIROSHI AOYAMA, IB-UNICAMP.
Ano de publicação:	2010
Referência:	Biometals, v. 23, n. 1, p. 93-98, Sep. 2010.
Conteúdo:	The in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirch- neriella subcapitata (formely Selenastrum capricor- nutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol -1 were determined in the absence and in the presence of 0.2 mM Cu2?, respectively. The dissociation constant value for Cu2? binding to the enzyme was determined to be 22.04 lM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu2? correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples.
Palavras-chave:	Ecotoxicologia
Digital Object Identifier:	10.1007/s10534-009-9270-z
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMA)