Título:	Analysis of the nucleotide sequence os the coat protein and 3: untranslated region of two Brazilian Potato Virus Y isolate
Autoria:	INOUE-NAGATA, A.K. FONSECA, M. E. N. LOBO, O. T. A. AVILA, A. C. de MONTE, D. C.
Afiliação:	EMBRAPA-CNPH, Brasilia, DF.
Ano de publicação:	2001
Referência:	Fitopatologia Brasileira, Brasilia, v.26, n.1, p.45-52, mar. 2001.
Conteúdo:	Two Brazilian Potato virus Y (PVY) isolates were biologically characterized as necrotic (PVY-NBR) and common (PVY-OBR) based upon symptoms on test plants. Additional characterization was performed by sequencing a cDNA corresponding to the 3’ terminal region of the viral genome. The sequence consisted of 195 nucleotides (nt) coding part of the nuclear inclusion body b (NIb) gene, 804 nt of the coat protein (CP) gene, and 328 nt (PVY-OBR) or 326 nt (PVY-NBR) of the 3’-untranslated region (UTR). Translation of the sequence resulted in one single open reading frame with part of the NIb and a CP of 267 amino acids. The two isolates shared 95.1% similarity in the CP amino acid sequence. The CP and the 3’-UTR sequence of the Brazilian isolates were compared to those of other PVY isolates previously reported and unrooted phylogenetic trees were constructed. The trees revealed a separation of two distinct clusters, one comprising most of the common strains and the other comprising the necrotic strains. PVY-OBR was clustered in the common group and PVY-NBR in the necrotic one.
Thesagro:	Taxonomia Vírus
NAL Thesaurus:	Potyvirus taxonomy
Palavras-chave:	Sequencia de nucleotideos Caracterizacao PVY Characterization Nucleotide sequence
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPH)